Mass: 14659.539 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Synechococcus elongatus (bacteria) / Strain: ATCC 27144 / PCC 6301 / SAUG 1402/1 / Gene: syc0711_d / Production host: Escherichia coli (E. coli) / References: UniProt: Q5N468, UniProt: A0A0H3K708*PLUS
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
1
2D 1H-13C HSQC
1
3
1
3D HNCO
1
4
1
3DCBCA(CO)NH
1
5
1
3D HN(CA)CB
1
6
1
3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY
1
7
2
2D 1H-13C HSQC
1
8
1
3DHBHA(CO)NH
1
9
1
3D (H)CCH-COSY
1
10
1
3D HNCA
1
11
1
3DHN(CO)CA
1
12
1
3DHN(CA)CO
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.89 mM [U-100% 13C; U-100% 15N] SnR212.015, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 100 mM NaCL, 1 x Proteinase Inhibitors, 20 mM MES pH 6.5, 10 % D2O, 50 uM DSS, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.67mM [U-5% 13C; U-100% 15N] SnR212.015, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 100 mM NaCL, 1 x Proteinase Inhibitors, 20 mM MES pH 6.5, 10 % D2O, 50 uM DSS, 90% H2O/10% D2O