- PDB-2lnu: Solution NMR Structure of the uncharacterized protein from gene l... -
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Basic information
Entry
Database: PDB / ID: 2lnu
Title
Solution NMR Structure of the uncharacterized protein from gene locus rrnAC0354 of Haloarcula marismortui, Northeast Structural Genomics Consortium Target HmR11
Components
Uncharacterized protein
Keywords
Structural Genomics / Unknown Function / NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) / PSI-Biology / Protein Structure Initiative
Function / homology
Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #1680 / Protein of unknown function DUF1684 / Protein of unknown function (DUF1684) / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / Helix non-globular / Special / DUF1684 domain-containing protein
Mass: 22090.523 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Haloarcula marismortui (Halophile) / Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809 / Gene: rrnAC0354 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)pMgK / References: UniProt: Q5V502
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
1
2D 1H-13C HSQC
1
3
1
3D HNCO
1
4
1
3DCBCA(CO)NH
1
5
1
3D HN(CA)CB
1
6
1
3D 1H-13C arom NOESY
1
7
1
3D 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY
1
8
2
2Dhetnoe
1
9
2
1DT1inv.rec
1
10
2
1D T2 CPMG
1
11
2
2D 1H-13C HSQC highres
1
12
3
Jmod 15N TROSY
1
13
3
jmod 15N TROSY
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.7 mM [U-100% 13C; U-100% 15N] HmR11, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 100 mM NaCL, 1 x Proteinase Inhibitors, 20 mM MES pH 6.5, 10 % D2O, 50 uM DSS, 95% H2O/5% D2O
95% H2O/5% D2O
2
1.2 mM [U-5% 13C; U-100% 15N] HmR11, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 100 mM NaCL, 1 x Proteinase Inhibitors, 20 mM MES pH 6.5, 10 % D2O, 50 uM DSS, 95% H2O/5% D2O
95% H2O/5% D2O
3
0.75 mM [U-5% 13C; U-100% 15N] HmR11, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 100 mM NaCL, 1 x Proteinase Inhibitors, 20 mM MES pH 6.5, 10 % D2O, 50 uM DSS, 95% H2O/5% D2O