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Yorodumi- PDB-2kw5: Solution NMR Structure of the Slr1183 protein from Synechocystis ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2kw5 | ||||||
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Title | Solution NMR Structure of the Slr1183 protein from Synechocystis sp. PCC 6803, Northeast Structural Genomics Consortium Target SgR145 | ||||||
Components | Slr1183 protein | ||||||
Keywords | Structural Genomics / Unknown function / NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) / PSI-2 / Protein Structure Initiative | ||||||
Function / homology | Methyltransferase domain 25 / Methyltransferase domain / Vaccinia Virus protein VP39 / transferase activity / S-adenosyl-L-methionine-dependent methyltransferase superfamily / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta / Slr1183 protein Function and homology information | ||||||
Biological species | Synechocystis (bacteria) | ||||||
Method | SOLUTION NMR | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Rossi, P. / Forouhar, F. / Lee, H. / Lange, O. / Mao, B. / Lemak, A. / Maglaqui, M. / Belote, R. / Ciccosanti, C. / Foote, E. ...Rossi, P. / Forouhar, F. / Lee, H. / Lange, O. / Mao, B. / Lemak, A. / Maglaqui, M. / Belote, R. / Ciccosanti, C. / Foote, E. / Sahdev, S. / Acton, T. / Xiao, R. / Everett, J. / Baker, D. / Montelione, G.T. / Northeast Structural Genomics Consortium (NESG) | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2012 Title: Determination of solution structures of proteins up to 40 kDa using CS-Rosetta with sparse NMR data from deuterated samples. Authors: Lange, O.F. / Rossi, P. / Sgourakis, N.G. / Song, Y. / Lee, H.W. / Aramini, J.M. / Ertekin, A. / Xiao, R. / Acton, T.B. / Montelione, G.T. / Baker, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kw5.cif.gz | 1.2 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2kw5.ent.gz | 1 MB | Display | PDB format |
PDBx/mmJSON format | 2kw5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kw/2kw5 ftp://data.pdbj.org/pub/pdb/validation_reports/kw/2kw5 | HTTPS FTP |
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-Related structure data
Related structure data | 2kznC 2lmdC 2lnuC 2lokC 2loyC 2mv0C C: citing same article (ref.) |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 22403.258 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Synechocystis (bacteria) / Strain: PCC 6803 / Gene: slr1183 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: P74712 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.1 mM [U-100% 13C; U-100% 15N] SgR145, 95% H2O/5% D2O Solvent system: 95% H2O/5% D2O |
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Sample | Conc.: 1.1 mM / Component: SgR145-1 / Isotopic labeling: [U-100% 13C; U-100% 15N] |
Sample conditions | pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |