[English] 日本語
![](img/lk-miru.gif)
- PDB-2lmc: Structure of T7 transcription factor Gp2-E. coli RNAp jaw domain ... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 2lmc | ||||||
---|---|---|---|---|---|---|---|
Title | Structure of T7 transcription factor Gp2-E. coli RNAp jaw domain complex | ||||||
![]() |
| ||||||
![]() | TRANSCRIPTION / TRANSFERASE | ||||||
Function / homology | ![]() symbiont-mediated suppression of host transcription / submerged biofilm formation / cellular response to cell envelope stress / cytosolic DNA-directed RNA polymerase complex / regulation of DNA-templated transcription initiation / bacterial-type flagellum assembly / bacterial-type flagellum-dependent cell motility / nitrate assimilation / transcription elongation factor complex / regulation of DNA-templated transcription elongation ...symbiont-mediated suppression of host transcription / submerged biofilm formation / cellular response to cell envelope stress / cytosolic DNA-directed RNA polymerase complex / regulation of DNA-templated transcription initiation / bacterial-type flagellum assembly / bacterial-type flagellum-dependent cell motility / nitrate assimilation / transcription elongation factor complex / regulation of DNA-templated transcription elongation / transcription antitermination / DNA-templated transcription initiation / cell motility / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / response to heat / intracellular iron ion homeostasis / response to antibiotic / magnesium ion binding / DNA binding / zinc ion binding / membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | minimized average structure, model 1 | ||||||
Model type details | minimized average | ||||||
![]() | Liu, M. | ||||||
![]() | ![]() Title: Structural and Mechanistic Basis for the Inhibition of Escherichia coli RNA Polymerase by T7 Gp2. Authors: James, E. / Liu, M. / Sheppard, C. / Mekler, V. / Camara, B. / Liu, B. / Simpson, P. / Cota, E. / Severinov, K. / Matthews, S. / Wigneshweraraj, S. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 372.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 307.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 425.7 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 549.7 KB | Display | |
Data in XML | ![]() | 21.7 KB | Display | |
Data in CIF | ![]() | 35.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
---|---|
Other databases |
-
Links
-
Assembly
Deposited unit | ![]()
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-
Components
#1: Protein | Mass: 9352.351 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
---|---|
#2: Protein | Mass: 9356.563 Da / Num. of mol.: 1 / Fragment: UNP residues 1151-1213 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
---|---|
NMR experiment | Type: 2D 1H-15N ![]() |
-
Sample preparation
Details | Contents: 300 mM [U-100% 13C; U-100% 15N] Gp2-Jaw complex, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
---|---|
Sample | Conc.: 300 mM / Component: Gp2-Jaw complex-1 / Isotopic labeling: [U-100% 13C; U-100% 15N] |
Sample conditions | Ionic strength: 0.05 / pH: 6.5 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
NMR spectrometer |
|
---|
-
Processing
NMR software |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: minimized average structure | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 |