+Open data
-Basic information
Entry | Database: PDB / ID: 2lc6 | ||||||
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Title | Solution structure of Par-6 Q144C/L164C | ||||||
Components | Par-6 | ||||||
Keywords | CELL ADHESION / PDZ domain / CRIB / Cdc42 | ||||||
Function / homology | Function and homology information CDC42 GTPase cycle / RAC1 GTPase cycle / RHOV GTPase cycle / terminal branching, open tracheal system / subapical complex / establishment or maintenance of neuroblast polarity / branching involved in open tracheal system development / zonula adherens assembly / establishment of neuroblast polarity / RHOU GTPase cycle ...CDC42 GTPase cycle / RAC1 GTPase cycle / RHOV GTPase cycle / terminal branching, open tracheal system / subapical complex / establishment or maintenance of neuroblast polarity / branching involved in open tracheal system development / zonula adherens assembly / establishment of neuroblast polarity / RHOU GTPase cycle / TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) / Asymmetric localization of PCP proteins / establishment or maintenance of polarity of embryonic epithelium / muscle cell postsynaptic specialization / border follicle cell migration / asymmetric neuroblast division / morphogenesis of a polarized epithelium / apical cortex / apical protein localization / establishment or maintenance of epithelial cell apical/basal polarity / positive regulation of smoothened signaling pathway / centrosome cycle / positive regulation of filopodium assembly / protein kinase inhibitor activity / bicellular tight junction / positive regulation of lamellipodium assembly / synapse assembly / terminal bouton / cell cortex / apical plasma membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Volkman, B.F. / Whitney, D.S. / Peterson, F.C. | ||||||
Citation | Journal: Structure / Year: 2011 Title: A Conformational Switch in the CRIB-PDZ Module of Par-6. Authors: Whitney, D.S. / Peterson, F.C. / Volkman, B.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2lc6.cif.gz | 782.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2lc6.ent.gz | 665.4 KB | Display | PDB format |
PDBx/mmJSON format | 2lc6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lc/2lc6 ftp://data.pdbj.org/pub/pdb/validation_reports/lc/2lc6 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13724.749 Da / Num. of mol.: 1 / Fragment: sequence database residues 130-255 / Mutation: Q144C, L164C Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: CG5884, Dmel_CG5884, par-6 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: O97111 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1 mM [U-100% 13C; U-100% 15N] Par-6, 20 mM sodium phosphate, 50 mM sodium chloride, 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 53 / pH: 5.5 / Pressure: AMBIENT / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance II / Manufacturer: Bruker / Model: AVANCE II / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 Details: AUTOMATED METHODS WERE USED FOR BACKBONE CHEMICAL SHIFT ASSIGNMENT AND ITERATIVE NOE REFINEMENT. FINAL STRUCTURES WERE OBTAINED BY MOLECULAR DYNAMICS IN EXPLICIT SOLVENTSTRUCTURES ARE BASED ...Details: AUTOMATED METHODS WERE USED FOR BACKBONE CHEMICAL SHIFT ASSIGNMENT AND ITERATIVE NOE REFINEMENT. FINAL STRUCTURES WERE OBTAINED BY MOLECULAR DYNAMICS IN EXPLICIT SOLVENTSTRUCTURES ARE BASED ON A TOTAL OF 1604 NOE CONSTRAINTS ( 407 INTRA, 388 SEQUENTIAL, 251 MEDIUM, AND 558 LONG RANGE) AND 165 PHI AND PSI DIHEDRAL ANGLE CONSTRAINTS., STRUCTURES ARE BASED ON A TOTAL OF 1604 NOE CONSTRAINTS ( 407 INTRA, 388 SEQUENTIAL, 251 MEDIUM, AND 558 LONG RANGE) AND 165 PHI AND PSI DIHEDRAL ANGLE CONSTRAINTS. | ||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 1604 / NOE intraresidue total count: 407 / NOE long range total count: 558 / NOE medium range total count: 251 / NOE sequential total count: 388 / Protein phi angle constraints total count: 82 / Protein psi angle constraints total count: 83 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |