+Open data
-Basic information
Entry | Database: PDB / ID: 2kyc | ||||||
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Title | solution structure of Ca-free chicken parvalbumin 3 (CPV3) | ||||||
Components | Parvalbumin, thymic CPV3 | ||||||
Keywords | CALCIUM BINDING PROTEIN / EF-hand protein / parvalbumin | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Gallus gallus (chicken) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Model details | closest to the average, model 1 | ||||||
Authors | Henzl, N.T. / Tanner, J.J. / Tan, A. | ||||||
Citation | Journal: Proteins / Year: 2011 Title: Solution structures of chicken parvalbumin 3 in the Ca(2+) -free and Ca(2+) -bound states. Authors: Henzl, M.T. / Tanner, J.J. / Tan, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kyc.cif.gz | 639 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kyc.ent.gz | 535.2 KB | Display | PDB format |
PDBx/mmJSON format | 2kyc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ky/2kyc ftp://data.pdbj.org/pub/pdb/validation_reports/ky/2kyc | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11972.276 Da / Num. of mol.: 1 / Mutation: C73S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Gallus gallus (chicken) / Plasmid: pET11 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P43305 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 3.0 mM [U-98% 13C; U-98% 15N] ca-free CPV3, 150 mM sodium chloride, 10 mM MES, 10 % D2O, 0.1 % sodium azide, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 0.165 / pH: 6.0 / Pressure: AMBIENT / Temperature: 293 K |
-NMR measurement
NMR spectrometer | Type: Varian Inova / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |