- PDB-2kvo: Solution NMR structure of Photosystem II reaction center Psb28 pr... -
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基本情報
登録情報
データベース: PDB / ID: 2kvo
タイトル
Solution NMR structure of Photosystem II reaction center Psb28 protein from Synechocystis sp.(strain PCC 6803), Northeast Structural Genomics Consortium Target SgR171
要素
Photosystem II reaction center psb28 protein
キーワード
PHOTOSYNTHESIS / Photosystem II reaction center Psb28 protein / Membrane / Photosystem II / Thylakoid / Structural Genomics / PSI-2 / Protein Structure Initiative / Northeast Structural Genomics Consortium / NESG
Photosystem II Psb28 / Photosystem II Psb28, class 1 / Photosystem II Psb28, class 1 superfamily / Psb28 protein / Elongation Factor Tu (Ef-tu); domain 3 / Beta Barrel / Mainly Beta 類似検索 - ドメイン・相同性
Photosystem II reaction center Psb28 protein 類似検索 - 構成要素
手法: 溶液NMR 詳細: Solution NMR structure Photosystem II reaction center Psb28 protein Synechocystis sp.(strain PCC 6803) Northeast Structural Genomics Consortium (NESG)
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
2D 1H-15N HSQC
1
2
2
2D 1H-13C HSQC CT
1
3
1
3D 1H-15N NOESY
1
4
1
3D 1H-13C NOESY
1
5
1
3D HNCO
1
6
1
3D HNCA
1
7
1
3D HN(CA)CB
1
8
1
3DCBCA(CO)NH
1
9
1
3DHN(CO)CA
1
10
1
3DHBHA(CO)NH
1
11
1
3DH(CCO)NH
1
12
1
3DC(CO)NH
1
13
1
3D (H)CCH-TOCSY
1
14
1
3D (H)CCH-COSY
1
15
3
3D (H)CCH-TOCSY
1
16
3
4D CC NOESY
1
17
1
2D 1H-15N HSQC swN150ppm
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試料調製
詳細
Solution-ID
内容
溶媒系
1
0.58 mM [U-100% 13C; U-100% 15N] Photosystem II reaction center Psb28 protein from Synechocystis sp.(strain PCC 6803), 20 mM MES, 100 mM sodium chloride, 5 mM calcium chloride, 10 mM DTT, 0.02 % sodium azide, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.44 mM [U-5% 13C; U-100% 15N] Photosystem II reaction center Psb28 protein from Synechocystis sp.(strain PCC 6803), 20 mM MES, 100 mM sodium chloride, 5 mM calcium chloride, 10 mM DTT, 0.02 % sodium azide, 90% H2O/10% D2O
90% H2O/10% D2O
3
0.58 mM [U-100% 13C; U-100% 15N] Photosystem II reaction center Psb28 protein from Synechocystis sp.(strain PCC 6803), 20 mM MES, 100 mM sodium chloride, 5 mM calcium chloride, 10 mM DTT, 0.02 % sodium azide, 100% D2O
100% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
0.58mM
Photosystem II reaction center Psb28 protein from Synechocystis sp.(strain PCC 6803)-1
[U-100% 13C; U-100% 15N]
1
20mM
MES-2
1
100mM
sodium chloride-3
1
5mM
calcium chloride-4
1
10mM
DTT-5
1
0.02 %
sodium azide-6
1
0.44mM
Photosystem II reaction center Psb28 protein from Synechocystis sp.(strain PCC 6803)-7
[U-5% 13C; U-100% 15N]
2
20mM
MES-8
2
100mM
sodium chloride-9
2
5mM
calcium chloride-10
2
10mM
DTT-11
2
0.02 %
sodium azide-12
2
0.58mM
Photosystem II reaction center Psb28 protein from Synechocystis sp.(strain PCC 6803)-13
[U-100% 13C; U-100% 15N]
3
20mM
MES-14
3
100mM
sodium chloride-15
3
5mM
calcium chloride-16
3
10mM
DTT-17
3
0.02 %
sodium azide-18
3
試料状態
イオン強度: 0.2 / pH: 6.5 / 圧: ambient / 温度: 293 K
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NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Varian INOVA
Varian
INOVA
600
1
Bruker AvanceIII
Bruker
AVANCEIII
850
2
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解析
NMR software
名称
バージョン
開発者
分類
NMRPipe
2008
Delaglio, Grzesiek, Vuister, Zhu, PfeiferandBax
解析
VNMR
6.1C
Varian
collection
TopSpin
2.1.4
BrukerBiospin
collection
AutoStructure
2.2.1
Huang, Tejero, PowersandMontelione
データ解析
X-PLOR NIH
2.2
Schwieters, Kuszewski, TjandraandClore
構造決定
CNS
1.2
Brunger, Adams, Clore, Gros, NilgesandRead
精密化
Sparky
3.113
Goddard
データ解析
PSVS
1.4
BhattacharyaandMontelione
精密化
AutoAssign
2.3
Zimmerman, Moseley, KulikowskiandMontelione
chemicalshiftassignment
PdbStat
5.1
(PdbStat)-Roberto Tejero and Gaetano T. Montelione
構造決定
PINE Server
1
Bahrami, A., Assadi, A., Markley, J. L. & Eghbalnia, H.