- PDB-2kvo: Solution NMR structure of Photosystem II reaction center Psb28 pr... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 2kvo
Title
Solution NMR structure of Photosystem II reaction center Psb28 protein from Synechocystis sp.(strain PCC 6803), Northeast Structural Genomics Consortium Target SgR171
Components
Photosystem II reaction center psb28 protein
Keywords
PHOTOSYNTHESIS / Photosystem II reaction center Psb28 protein / Membrane / Photosystem II / Thylakoid / Structural Genomics / PSI-2 / Protein Structure Initiative / Northeast Structural Genomics Consortium / NESG
Function / homology
Function and homology information
plasma membrane-derived thylakoid photosystem II / photosystem II oxygen evolving complex / plasma membrane-derived thylakoid membrane / photosynthesis Similarity search - Function
Photosystem II Psb28 / Photosystem II Psb28, class 1 / Photosystem II Psb28, class 1 superfamily / Psb28 protein / Elongation Factor Tu (Ef-tu); domain 3 / Beta Barrel / Mainly Beta Similarity search - Domain/homology
PhotosystemIIreactioncenterpsb28protein / Photosystem II reaction center W protein / Photosystem II 13 kDa protein
Mass: 13675.436 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Synechocystis sp. (bacteria) / Strain: PCC 6803 / Description: C-tag: LEHHHHHH / Gene: psb28, psbW, sll1398 / Plasmid: pET 21-23C / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) pMGK / References: UniProt: Q55356
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR Details: Solution NMR structure Photosystem II reaction center Psb28 protein Synechocystis sp.(strain PCC 6803) Northeast Structural Genomics Consortium (NESG)
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
2
2D 1H-13C HSQC CT
1
3
1
3D 1H-15N NOESY
1
4
1
3D 1H-13C NOESY
1
5
1
3D HNCO
1
6
1
3D HNCA
1
7
1
3D HN(CA)CB
1
8
1
3DCBCA(CO)NH
1
9
1
3DHN(CO)CA
1
10
1
3DHBHA(CO)NH
1
11
1
3DH(CCO)NH
1
12
1
3DC(CO)NH
1
13
1
3D (H)CCH-TOCSY
1
14
1
3D (H)CCH-COSY
1
15
3
3D (H)CCH-TOCSY
1
16
3
4D CC NOESY
1
17
1
2D 1H-15N HSQC swN150ppm
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.58 mM [U-100% 13C; U-100% 15N] Photosystem II reaction center Psb28 protein from Synechocystis sp.(strain PCC 6803), 20 mM MES, 100 mM sodium chloride, 5 mM calcium chloride, 10 mM DTT, 0.02 % sodium azide, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.44 mM [U-5% 13C; U-100% 15N] Photosystem II reaction center Psb28 protein from Synechocystis sp.(strain PCC 6803), 20 mM MES, 100 mM sodium chloride, 5 mM calcium chloride, 10 mM DTT, 0.02 % sodium azide, 90% H2O/10% D2O
90% H2O/10% D2O
3
0.58 mM [U-100% 13C; U-100% 15N] Photosystem II reaction center Psb28 protein from Synechocystis sp.(strain PCC 6803), 20 mM MES, 100 mM sodium chloride, 5 mM calcium chloride, 10 mM DTT, 0.02 % sodium azide, 100% D2O
100% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
0.58mM
Photosystem II reaction center Psb28 protein from Synechocystis sp.(strain PCC 6803)-1
[U-100% 13C; U-100% 15N]
1
20mM
MES-2
1
100mM
sodium chloride-3
1
5mM
calcium chloride-4
1
10mM
DTT-5
1
0.02 %
sodium azide-6
1
0.44mM
Photosystem II reaction center Psb28 protein from Synechocystis sp.(strain PCC 6803)-7
[U-5% 13C; U-100% 15N]
2
20mM
MES-8
2
100mM
sodium chloride-9
2
5mM
calcium chloride-10
2
10mM
DTT-11
2
0.02 %
sodium azide-12
2
0.58mM
Photosystem II reaction center Psb28 protein from Synechocystis sp.(strain PCC 6803)-13
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