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Open data
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Basic information
Entry | Database: PDB / ID: 2kra | ||||||
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Title | Solution structure of Bv8 | ||||||
![]() | Prokineticin Bv8 | ||||||
![]() | CONTRACTILE PROTEIN / beta strands / beta turn / helix / Disulfide bond / Secreted | ||||||
Function / homology | ![]() | ||||||
Biological species | Bombina variegata (yellow-bellied toad) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Model details | lowest energy, model 1 | ||||||
![]() | Daly, N.L. / Craik, D.J. / Mobli, M. | ||||||
![]() | ![]() Title: Chemical synthesis and structure of the prokineticin Bv8 Authors: Morales, R.A.V. / Daly, N.L. / Vetter, I. / Mobli, M. / Napier, I.A. / Craik, D.J. / Lewis, R.J. / Christie, M.J. / King, G.F. / Alewood, P.F. / Durek, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 418.4 KB | Display | ![]() |
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PDB format | ![]() | 363.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 337.6 KB | Display | ![]() |
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Full document | ![]() | 441.3 KB | Display | |
Data in XML | ![]() | 26.6 KB | Display | |
Data in CIF | ![]() | 43.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 8042.226 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bombina variegata (yellow-bellied toad) Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 1mM Bv8, 90% H2O/10% D2O / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 1 mM / Component: Bv8 |
Sample conditions | Ionic strength: 0 / pH: 3.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: Avance / Field strength: 900 MHz |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 20 / Representative conformer: 1 |