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Open data
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Basic information
Entry | Database: PDB / ID: 2km0 | ||||||
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Title | Cu(I)-bound CopK | ||||||
![]() | Copper resistance protein K | ||||||
![]() | METAL BINDING PROTEIN / heavy metal resistance / periplasmic copper binding protein / Copper / Periplasm / Plasmid | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing, molecular dynamics | ||||||
Model details | lowest energy, model 1 | ||||||
![]() | Bersch, B. | ||||||
![]() | ![]() Title: CopK from Cupriavidus metallidurans CH34 Binds Cu(I) in a Tetrathioether Site: Characterization by X-ray Absorption and NMR Spectroscopy Authors: Sarret, G. / Favier, A. / Hazemann, J.L. / Mergeay, M. / Bersch, B. #1: ![]() Title: Molecular structure and metal-binding properties of the periplasmic CopK protein expressed in Cupriavidus metallidurans CH34 during copper challenge Authors: Bersch, B. / Favier, A. / Schanda, P. / van Aelst, S. / Vallaeys, T. / Coves, J. / Mergeay, M. / Wattiez, R. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 507.1 KB | Display | ![]() |
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PDB format | ![]() | 427.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 402.1 KB | Display | ![]() |
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Full document | ![]() | 550.9 KB | Display | |
Data in XML | ![]() | 29.2 KB | Display | |
Data in CIF | ![]() | 46 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Similar structure data | |
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 8294.567 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-CU1 / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR / Details: CopK protein bound to a single Cu(I) | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample |
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Sample conditions | pH: 6.8 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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Processing
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Refinement | Method: simulated annealing, molecular dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 1000 / Conformers submitted total number: 20 / Representative conformer: 1 |