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Yorodumi- PDB-2kky: Solution Structure of C-terminal domain of oxidized NleG2-3 (resi... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2kky | ||||||
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Title | Solution Structure of C-terminal domain of oxidized NleG2-3 (residue 90-191) from Pathogenic E. coli O157:H7. Northeast Structural Genomics Consortium and Midwest Center for Structural Genomics target ET109A | ||||||
Components | Uncharacterized protein ECs2156 | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / Methods Development / U-box domain / PSI-2 / Protein Structure Initiative / Northeast Structural Genomics Consortium / NESG / Midwest Center for Structural Genomics / MCSG / Ontario Centre for Structural Proteomics / OCSP | ||||||
Function / homology | Function and homology information biological process involved in symbiotic interaction / ubiquitin-protein transferase activity Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Wu, B. / Yee, A. / Fares, C. / Lemak, A. / Semest, A. / Claude, M. / Singer, A. / Edwards, A. / Savchenko, A. / Montelione, G.T. ...Wu, B. / Yee, A. / Fares, C. / Lemak, A. / Semest, A. / Claude, M. / Singer, A. / Edwards, A. / Savchenko, A. / Montelione, G.T. / Joachimiak, A. / Arrowsmith, C.H. / Northeast Structural Genomics Consortium (NESG) / Midwest Center for Structural Genomics (MCSG) / Ontario Centre for Structural Proteomics (OCSP) | ||||||
Citation | Journal: Plos Pathog. / Year: 2010 Title: NleG Type 3 effectors from enterohaemorrhagic Escherichia coli are U-Box E3 ubiquitin ligases. Authors: Wu, B. / Skarina, T. / Yee, A. / Jobin, M.C. / Dileo, R. / Semesi, A. / Fares, C. / Lemak, A. / Coombes, B.K. / Arrowsmith, C.H. / Singer, A.U. / Savchenko, A. #1: Journal: J.Biomol.Nmr / Year: 2011 Title: A novel strategy for NMR resonance assignment and protein structure determination. Authors: Lemak, A. / Gutmanas, A. / Chitayat, S. / Karra, M. / Fares, C. / Sunnerhagen, M. / Arrowsmith, C.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kky.cif.gz | 605 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kky.ent.gz | 509.1 KB | Display | PDB format |
PDBx/mmJSON format | 2kky.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2kky_validation.pdf.gz | 529.6 KB | Display | wwPDB validaton report |
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Full document | 2kky_full_validation.pdf.gz | 737.7 KB | Display | |
Data in XML | 2kky_validation.xml.gz | 45.2 KB | Display | |
Data in CIF | 2kky_validation.cif.gz | 68.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kk/2kky ftp://data.pdbj.org/pub/pdb/validation_reports/kk/2kky | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11290.848 Da / Num. of mol.: 1 / Fragment: residues 90-191 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: O157:H7 / Gene: ECs2156, Z2149 / Production host: Escherichia coli (E. coli) / References: UniProt: Q8X509 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR / Details: PSI Methods Development | ||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 300 / pH: 7 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |