+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2kb0 | ||||||
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タイトル | Cdc42(T35A) | ||||||
要素 | Cell division control protein 42 homolog | ||||||
キーワード | SIGNALING PROTEIN / globular / folded / Switch 1 / mutation / Ras / Alternative splicing / Cell membrane / GTP-binding / Lipoprotein / Membrane / Methylation / Nucleotide-binding / Prenylation | ||||||
機能・相同性 | 機能・相同性情報 GBD domain binding / submandibular salivary gland formation / actin filament branching / Golgi transport complex / positive regulation of epithelial cell proliferation involved in lung morphogenesis / positive regulation of pinocytosis / modification of synaptic structure / Cdc42 protein signal transduction / positive regulation of synapse structural plasticity / dendritic cell migration ...GBD domain binding / submandibular salivary gland formation / actin filament branching / Golgi transport complex / positive regulation of epithelial cell proliferation involved in lung morphogenesis / positive regulation of pinocytosis / modification of synaptic structure / Cdc42 protein signal transduction / positive regulation of synapse structural plasticity / dendritic cell migration / endothelin receptor signaling pathway involved in heart process / cardiac neural crest cell migration involved in outflow tract morphogenesis / positive regulation of pseudopodium assembly / storage vacuole / apolipoprotein A-I receptor binding / neuron fate determination / GTP-dependent protein binding / modulation by host of viral process / organelle transport along microtubule / regulation of attachment of spindle microtubules to kinetochore / cardiac conduction system development / Inactivation of CDC42 and RAC1 / establishment of Golgi localization / regulation of filopodium assembly / leading edge membrane / neuropilin signaling pathway / positive regulation of intracellular protein transport / cell junction assembly / filopodium assembly / establishment of epithelial cell apical/basal polarity / mitogen-activated protein kinase kinase kinase binding / dendritic spine morphogenesis / regulation of modification of postsynaptic structure / regulation of stress fiber assembly / thioesterase binding / embryonic heart tube development / RHO GTPases activate KTN1 / regulation of lamellipodium assembly / nuclear migration / adherens junction organization / DCC mediated attractive signaling / sprouting angiogenesis / CD28 dependent Vav1 pathway / Wnt signaling pathway, planar cell polarity pathway / positive regulation of filopodium assembly / regulation of postsynapse organization / regulation of mitotic nuclear division / RHOV GTPase cycle / establishment or maintenance of cell polarity / phagocytosis, engulfment / heart contraction / positive regulation of cytokinesis / Myogenesis / RHOJ GTPase cycle / Golgi organization / RHOQ GTPase cycle / RHO GTPases activate PAKs / RHOU GTPase cycle / macrophage differentiation / CDC42 GTPase cycle / RHOG GTPase cycle / RAC2 GTPase cycle / RHO GTPases Activate WASPs and WAVEs / RHO GTPases activate IQGAPs / spindle midzone / RAC3 GTPase cycle / negative regulation of protein-containing complex assembly / positive regulation of lamellipodium assembly / phagocytic vesicle / positive regulation of substrate adhesion-dependent cell spreading / positive regulation of stress fiber assembly / GPVI-mediated activation cascade / substantia nigra development / EPHB-mediated forward signaling / RAC1 GTPase cycle / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / small monomeric GTPase / G protein activity / actin filament organization / secretory granule / filopodium / positive regulation of DNA replication / integrin-mediated signaling pathway / regulation of actin cytoskeleton organization / RHO GTPases Activate Formins / FCGR3A-mediated phagocytosis / positive regulation of JNK cascade / EGFR downregulation / MAPK6/MAPK4 signaling / Schaffer collateral - CA1 synapse / cellular response to type II interferon / mitotic spindle / G beta:gamma signalling through CDC42 / Regulation of actin dynamics for phagocytic cup formation / VEGFA-VEGFR2 Pathway / cytoplasmic ribonucleoprotein granule / endocytosis / positive regulation of neuron apoptotic process / ubiquitin protein ligase activity / cell-cell junction 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / DGSA-distance geometry simulated annealing | ||||||
データ登録者 | Adams, P.D. / Oswald, R.E. / McFeeters, R. | ||||||
引用 | ジャーナル: To be Published タイトル: Cdc42(T35A) 著者: Adams, P. / Oswald, R. / McFeeters, R. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2kb0.cif.gz | 579.1 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2kb0.ent.gz | 481.6 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2kb0.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 2kb0_validation.pdf.gz | 344.5 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 2kb0_full_validation.pdf.gz | 463.9 KB | 表示 | |
XML形式データ | 2kb0_validation.xml.gz | 48.2 KB | 表示 | |
CIF形式データ | 2kb0_validation.cif.gz | 63 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/kb/2kb0 ftp://data.pdbj.org/pub/pdb/validation_reports/kb/2kb0 | HTTPS FTP |
-関連構造データ
類似構造データ | |
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その他のデータベース |
-リンク
-集合体
登録構造単位 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 19744.678 Da / 分子数: 1 / 変異: T35A / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: CDC42 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P60953 |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||
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NMR実験 |
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-試料調製
詳細 | 内容: 0.4 mM [U-100% 13C; U-100% 15N] Cdc42(T35A), 90% H2O/10% D2O 溶媒系: 90% H2O/10% D2O |
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試料 | 濃度: 0.4 mM / 構成要素: Cdc42(T35A) / Isotopic labeling: [U-100% 13C; U-100% 15N] |
試料状態 | pH: 5.5 / 圧: ambient / 温度: 298 K |
-NMR測定
NMRスペクトロメーター | タイプ: Varian AMX / 製造業者: Varian / モデル: AMX / 磁場強度: 500 MHz |
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-解析
NMR software |
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精密化 | 手法: DGSA-distance geometry simulated annealing / ソフトェア番号: 1 / 詳細: nih-xplor used to calculate structures | |||||||||
代表構造 | 選択基準: closest to the average | |||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 18 / 登録したコンフォーマーの数: 12 |