- PDB-2k17: Solution structure of the TAF3 PHD domain in complex with a H3K4m... -
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基本情報
登録情報
データベース: PDB / ID: 2k17
タイトル
Solution structure of the TAF3 PHD domain in complex with a H3K4me3 peptide
要素
H3K4me3 peptide
Transcription initiation factor TFIID subunit 3
キーワード
TRANSCRIPTION / PROTEIN / Alternative splicing / Metal-binding / Nucleus / Phosphoprotein / Transcription regulation / Zinc / Zinc-finger
機能・相同性
機能・相同性情報
RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Polymerase II Pre-transcription Events / Regulation of TP53 Activity through Phosphorylation / maintenance of protein location in nucleus / transcription regulator inhibitor activity / transcription factor TFIID complex / positive regulation of transcription initiation by RNA polymerase II ...RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Polymerase II Pre-transcription Events / Regulation of TP53 Activity through Phosphorylation / maintenance of protein location in nucleus / transcription regulator inhibitor activity / transcription factor TFIID complex / positive regulation of transcription initiation by RNA polymerase II / RNA polymerase II preinitiation complex assembly / male germ cell nucleus / mRNA transcription by RNA polymerase II / negative regulation of DNA-binding transcription factor activity / p53 binding / nuclear membrane / transcription by RNA polymerase II / protein heterodimerization activity / regulation of transcription by RNA polymerase II / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / nucleus / metal ion binding 類似検索 - 分子機能
solution structure of the planhomeodomain of TFIID sununit TAF3 with a peptide derived from the N ...solution structure of the planhomeodomain of TFIID sununit TAF3 with a peptide derived from the N terminal tail of histone H3 trimethylated at K4
手法: 溶液NMR 詳細: solution structure of the planhomeodomain of TFIID sununit TAF3 with a peptide derived from the N terminal tail of histone H3 trimethylated at K4
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
3D 1H-15N NOESY
1
2
1
3D 1H-13C NOESY
1
3
1
2D 1H-1H NOESY
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試料調製
詳細
内容: 0.4 mM [U-13C; U-15N] TAF3_PHD, 150 mM potassium chloride, 20 mM potassium phosphate, 0.01 mM ZnCl2, 95% H2O/5% D2O 溶媒系: 95% H2O/5% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
0.4mM
TAF3_PHD
[U-13C; U-15N]
1
150mM
potassiumchloride
1
20mM
potassiumphosphate
1
0.01mM
ZnCl2
1
試料状態
pH: 7 / 圧: ambient / 温度: 293 K
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NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker Avance
Bruker
AVANCE
900
1
Bruker Avance
Bruker
AVANCE
750
2
Bruker Avance
Bruker
AVANCE
700
3
Bruker Avance
Bruker
AVANCE
600
4
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解析
NMR software
名称
開発者
分類
CNS
Brunger, Adams, Clore, Gros, NilgesandRead
精密化
CYANA
Guntert, MumenthalerandWuthrich
構造決定
NMRPipe
Delaglio, Grzesiek, Vuister, Zhu, PfeiferandBax
解析
Sparky
Goddard
データ解析
精密化
手法: simulated annealing / ソフトェア番号: 1 詳細: refinement in explicit solvent using RECOORD protocol
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20