+Open data
-Basic information
Entry | Database: PDB / ID: 2apn | ||||||
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Title | hi1723 solution structure | ||||||
Components | Protein HI1723 | ||||||
Keywords | Structural Genomics / UNKNOWN FUNCTION / hi1723 solution structure / Structure 2 Function Project / S2F | ||||||
Function / homology | Function and homology information protein maturation by [4Fe-4S] cluster transfer / iron-sulfur cluster assembly / protein maturation / 2 iron, 2 sulfur cluster binding / 4 iron, 4 sulfur cluster binding / iron ion binding / structural molecule activity / cytosol Similarity search - Function | ||||||
Biological species | Haemophilus influenzae (bacteria) | ||||||
Method | SOLUTION NMR / CNS 1.1 | ||||||
Authors | He, Y. / Yeh, D.C. / Orban, J. / Structure 2 Function Project (S2F) | ||||||
Citation | Journal: To be Published Title: Solution NMR Structure of HI1723 From Haemophilus Influenzae Authors: He, Y. / Yeh, D.C. / Orban, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2apn.cif.gz | 657.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2apn.ent.gz | 545.7 KB | Display | PDB format |
PDBx/mmJSON format | 2apn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2apn_validation.pdf.gz | 342.7 KB | Display | wwPDB validaton report |
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Full document | 2apn_full_validation.pdf.gz | 521.3 KB | Display | |
Data in XML | 2apn_validation.xml.gz | 75.6 KB | Display | |
Data in CIF | 2apn_validation.cif.gz | 99.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ap/2apn ftp://data.pdbj.org/pub/pdb/validation_reports/ap/2apn | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12200.545 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Haemophilus influenzae (bacteria) / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P45344 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 50 mM potassium phosphate, 100 mM sodium chloride, 4 mM DTT, pH7, 90% H2O with 10% D2O Solvent system: 90% H2O with 10% D2O |
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Sample conditions | Ionic strength: 50 ~ 100 mM / pH: 7 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: CNS 1.1 / Software ordinal: 1 / Details: simulated annealing | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |