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- PDB-2jse: NMR reveals absence of hydrogen bonding in adjacent UU and AG mis... -
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Entry | Database: PDB / ID: 2jse | ||||||||||||||||||||
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Title | NMR reveals absence of hydrogen bonding in adjacent UU and AG mismatches in an isolated internal loop from ribosomal RNA. | ||||||||||||||||||||
![]() | RNA (5'-R(*![]() ![]() ![]() Function / homology | ![]() ![]() Method | ![]() ![]() ![]() Model details | NMR structure of RNA loop 5' GUGG 3' / 3' CUAC 5' | ![]() Shankar, N. | ![]() ![]() Title: NMR Reveals the Absence of Hydrogen Bonding in Adjacent UU and AG Mismatches in an Isolated Internal Loop from Ribosomal RNA Authors: Shankar, N. / Xia, T. / Kennedy, S.D. / Krugh, T.R. / Mathews, D.H. / Turner, D.H. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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PDBx/mmCIF format | ![]() | 139.1 KB | Display | ![]() |
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PDB format | ![]() | 115.8 KB | Display | ![]() |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Assembly
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NMR ensembles |
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Components
#1: RNA chain | Mass: 7112.306 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: T7 polymerase used to transcribe the RNA from a synthetic duplex DNA template |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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NMR experiment | Type![]() ![]() |
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Sample preparation
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Sample |
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Sample conditions | Ionic strength: 80 / pH: 6 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: ![]() ![]() | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy and least distance and angle violations Conformers calculated total number: 20 / Conformers submitted total number: 10 |