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Open data
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Basic information
Entry | Database: PDB / ID: 6n8h | |||||||||||||||||||||||||||||||
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Title | RNA Duplex containing the internal loop 5'-GCAU/3'-UACG | |||||||||||||||||||||||||||||||
![]() | RNA (5'-R(*![]() RNA / Duplex / Internal loop / GU Wobble / CA pair | Function / homology | RNA | ![]() Biological species | unidentified (others) | Method | SOLUTION NMR / simulated annealing | ![]() Berger, K.D. / Kennedy, S.D. / Turner, D.H. | Funding support | | ![]()
![]() ![]() Title: Nuclear Magnetic Resonance Reveals That GU Base Pairs Flanking Internal Loops Can Adopt Diverse Structures. Authors: Berger, K.D. / Kennedy, S.D. / Turner, D.H. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 255.8 KB | Display | ![]() |
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PDB format | ![]() | 212.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 335.9 KB | Display | ![]() |
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Full document | ![]() | 435.1 KB | Display | |
Data in XML | ![]() | 8.8 KB | Display | |
Data in CIF | ![]() | 14.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6n8fC ![]() 6n8iC C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: RNA chain | Mass: 3191.964 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) unidentified (others) |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution Contents: 1 mM RNA (5'-R(*CP*GP*GP*GP*CP*AP*UP*CP*CP*G)-3'), 90% H2O/10% D2O Label: Natural abundance / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 1 mM / Component: RNA (5'-R(*CP*GP*GP*GP*CP*AP*UP*CP*CP*G)-3') / Isotopic labeling: none |
Sample conditions | Ionic strength: 130 Na+ mM / Label: conditions_1 / pH: 6.2 / Pressure: 1 atm / Temperature: 288 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | |||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |