Uncharacterised protein family UPF0352 / YejL-like superfamily / Protein of unknown function (DUF1414) / YejL-like / YejL-like / Orthogonal Bundle / Mainly Alpha / UPF0352 protein VP2129
手法: 溶液NMR / 詳細: homodimer. All helical. domain swapped dimer.
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
2D 1H-15N HSQC
1
2
1
2D 1H-13C HSQC
1
3
1
3D 1H-15N NOESY
1
4
1
3D 1H-13C NOESY aliph
1
5
1
3D HN(CA)CB
1
6
2
2D 1H-13C HSQC
1
7
3
3D 1H-13C NOESY
1
8
3
4D 1H-13C NOESY
1
9
1
3DHBHA(CO)NH
1
10
1
3D HNHA
1
11
1
3DCBCA(CO)NH
1
12
1
3D HNCA
1
13
1
3DHN(CO)CA
1
14
1
3D HNCO
1
15
1
3DC(CO)NH
1
16
1
3D (H)CCH-COSY
1
17
1
3D (H)CCH-TOCSY
1
18
3
2D 1H-13C HSQC
1
19
3
2D 1H-15N HSQC
1
20
4
3D edited filtered 1H-13C NOESY
1
21
1
3D 1H-13C NOESY arom
-
試料調製
詳細
Solution-ID
内容
溶媒系
1
1.4 mM [U-13C; U-15N] VP2129, 100 mM sodium chloride, 5 mM calcium chloride, 20 mM ammonium acetate, 10 mM DTT, 0.02 % sodium azide, 95% H2O/5% D2O
95% H2O/5% D2O
2
1.4 mM [U-5% 13C; U-100% 15N] VP2129, 100 mM sodium chloride, 5 mM calcium chloride, 20 mM ammonium acetate, 10 mM DTT, 0.02 % sodium azide, 95% H2O/5% D2O
95% H2O/5% D2O
3
1.4 mM [U-13C; U-15N] VP2129, 100 mM sodium chloride, 5 mM calcium chloride, 20 mM ammonium acetate, 10 mM DTT, 0.02 % sodium azide, 100% D2O
100% D2O
4
0.4 mM [U-13C; U-15N] VP2129, 100 mM sodium chloride, 5 mM calcium chloride, 20 mM ammonium acetate, 10 mM DTT, 0.02 % sodium azide, 0.4 mM VP2129, 100% D2O