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Yorodumi- PDB-2jo9: Mouse Itch 3rd WW domain complex with the Epstein-Barr virus late... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2jo9 | ||||||
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Title | Mouse Itch 3rd WW domain complex with the Epstein-Barr virus latent membrane protein 2A derived peptide EEPPPPYED | ||||||
Components |
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Keywords | LIGASE / Itch / WW / complex / Epstein-Barr virus / LMP2A | ||||||
Function / homology | Function and homology information regulation of protein deubiquitination / Downregulation of ERBB4 signaling / negative regulation of cytoplasmic pattern recognition receptor signaling pathway / Activated NOTCH1 Transmits Signal to the Nucleus / host cell endomembrane system / Regulation of necroptotic cell death / viral latency / negative regulation of defense response to virus / Degradation of GLI1 by the proteasome / protein K29-linked ubiquitination ...regulation of protein deubiquitination / Downregulation of ERBB4 signaling / negative regulation of cytoplasmic pattern recognition receptor signaling pathway / Activated NOTCH1 Transmits Signal to the Nucleus / host cell endomembrane system / Regulation of necroptotic cell death / viral latency / negative regulation of defense response to virus / Degradation of GLI1 by the proteasome / protein K29-linked ubiquitination / Hedgehog 'on' state / T cell anergy / positive regulation of T cell anergy / RUNX1 regulates transcription of genes involved in differentiation of HSCs / CXCR chemokine receptor binding / CD4-positive, alpha-beta T cell proliferation / NOD1/2 Signaling Pathway / Antigen processing: Ubiquitination & Proteasome degradation / negative regulation of CD4-positive, alpha-beta T cell proliferation / negative regulation of JNK cascade / HECT-type E3 ubiquitin transferase / arrestin family protein binding / positive regulation of receptor catabolic process / negative regulation of NF-kappaB transcription factor activity / ubiquitin-like protein ligase binding / protein monoubiquitination / ligase activity / protein K63-linked ubiquitination / protein autoubiquitination / protein K48-linked ubiquitination / ribonucleoprotein complex binding / ubiquitin ligase complex / protein catabolic process / receptor internalization / protein polyubiquitination / positive regulation of protein catabolic process / ubiquitin-protein transferase activity / ubiquitin protein ligase activity / cell cortex / early endosome membrane / cytoplasmic vesicle / ubiquitin-dependent protein catabolic process / symbiont-mediated perturbation of host ubiquitin-like protein modification / proteasome-mediated ubiquitin-dependent protein catabolic process / defense response to virus / host cell perinuclear region of cytoplasm / protein ubiquitination / innate immune response / apoptotic process / negative regulation of apoptotic process / host cell plasma membrane / nucleoplasm / membrane / nucleus / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Macias, M.J. / Shaw, A.Z. / Martin-Malpartida, P. / Morales, B. / Ruiz, L. / Ramirez-Espain, X. / Yraola, F. / Royo, M. | ||||||
Citation | Journal: Structure / Year: 2007 Title: NMR Structural Studies of the ItchWW3 Domain Reveal that Phosphorylation at T30 Inhibits the Interaction with PPxY-Containing Ligands Authors: Morales, B. / Ramirez-Espain, X. / Shaw, A.Z. / Martin-Malpartida, P. / Yraola, F. / Sanchez-Tillo, E. / Farrera, C. / Celada, A. / Royo, M. / Macias, M.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2jo9.cif.gz | 133.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2jo9.ent.gz | 107.5 KB | Display | PDB format |
PDBx/mmJSON format | 2jo9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2jo9_validation.pdf.gz | 352.7 KB | Display | wwPDB validaton report |
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Full document | 2jo9_full_validation.pdf.gz | 446.9 KB | Display | |
Data in XML | 2jo9_validation.xml.gz | 8.3 KB | Display | |
Data in CIF | 2jo9_validation.cif.gz | 13.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jo/2jo9 ftp://data.pdbj.org/pub/pdb/validation_reports/jo/2jo9 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 4276.708 Da / Num. of mol.: 1 / Fragment: WW 3 DOMAIN, SEQUENCE DATABASE RESIDUES 399-432 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Itch / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 References: UniProt: Q8C863, Ligases; Forming carbon-nitrogen bonds; Acid-amino-acid ligases (peptide synthases) |
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#2: Protein/peptide | Mass: 1072.078 Da / Num. of mol.: 1 / Fragment: SEQUENCE DATABASE RESIDUES 54-62 / Source method: obtained synthetically Details: The peptide was chemically syntheized. The sequence of the peptide is naturally found in Epstein-Barr virus. References: UniProt: P13285 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.4 / pH: 6.2 / Pressure: ambient / Temperature: 285 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 / Details: Aria 1.2 software used, all NOEs manually assigned | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 |