+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2jmf | ||||||
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タイトル | Solution structure of the Su(dx) WW4- Notch PY peptide complex | ||||||
要素 |
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キーワード | Ligase/Signaling Protein / WW domain / Notch / NMR solution / complex / Ligase-Signaling Protein COMPLEX | ||||||
機能・相同性 | 機能・相同性情報 pupal development / response to symbiont / neuroblast fate specification / epithelial cell type specification, open tracheal system / lamellocyte differentiation / regulation of cardioblast cell fate specification / positive regulation of crystal cell differentiation / negative regulation of compound eye photoreceptor development / R1/R6 cell differentiation / formation of a compartment boundary ...pupal development / response to symbiont / neuroblast fate specification / epithelial cell type specification, open tracheal system / lamellocyte differentiation / regulation of cardioblast cell fate specification / positive regulation of crystal cell differentiation / negative regulation of compound eye photoreceptor development / R1/R6 cell differentiation / formation of a compartment boundary / compartment boundary maintenance / RHOQ GTPase cycle / follicle cell of egg chamber migration / muscle cell fate determination / imaginal disc-derived leg joint morphogenesis / eye-antennal disc development / crystal cell differentiation / R8 cell development / R7 cell differentiation / R3/R4 cell differentiation / germ-line stem-cell niche homeostasis / oocyte localization involved in germarium-derived egg chamber formation / hemocyte proliferation / negative regulation of lamellocyte differentiation / compound eye retinal cell programmed cell death / myoblast development / imaginal disc-derived male genitalia morphogenesis / germarium-derived egg chamber formation / leg disc morphogenesis / epithelial cell proliferation involved in Malpighian tubule morphogenesis / CSL-Notch-Mastermind transcription factor complex / lateral inhibition / Malpighian tubule tip cell differentiation / eye-antennal disc morphogenesis / second mitotic wave involved in compound eye morphogenesis / imaginal disc-derived wing margin morphogenesis / Activated NOTCH1 Transmits Signal to the Nucleus / imaginal disc-derived leg segmentation / RUNX1 regulates transcription of genes involved in differentiation of HSCs / sensory organ precursor cell fate determination / female germ-line stem cell population maintenance / ommatidial rotation / wing disc dorsal/ventral pattern formation / Downregulation of ERBB4 signaling / RHOU GTPase cycle / defense response to insect / wing disc pattern formation / imaginal disc-derived wing vein morphogenesis / Regulation of PTEN stability and activity / follicle cell of egg chamber stalk formation / imaginal disc-derived leg morphogenesis / chaeta morphogenesis / dorsal closure / compound eye morphogenesis / Antigen processing: Ubiquitination & Proteasome degradation / follicle cell of egg chamber development / larval lymph gland hemopoiesis / neuroblast development / lymph gland development / cell dedifferentiation / neuroblast fate determination / imaginal disc-derived wing vein specification / dorsal appendage formation / border follicle cell migration / intestinal stem cell homeostasis / compound eye development / chaeta development / foregut morphogenesis / asymmetric cell division / regulation of stem cell division / imaginal disc-derived wing morphogenesis / glial cell fate determination / neuron fate specification / regulation of neuroblast proliferation / retinal cell programmed cell death / glial cell migration / sensory organ development / germ-line stem cell population maintenance / dorsal/ventral axis specification / neuron fate determination / WW domain binding / glial cell differentiation / neuronal stem cell population maintenance / regulation of filopodium assembly / lipid storage / protein targeting to lysosome / negative regulation of cell-cell adhesion mediated by cadherin / peripheral nervous system development / HECT-type E3 ubiquitin transferase / motor neuron axon guidance / nervous system process / Notch binding / regulation of growth / regulation of glycolytic process / embryonic hemopoiesis / histone acetyltransferase binding / muscle cell cellular homeostasis / morphogenesis of an epithelial fold / positive regulation of neuroblast proliferation / oogenesis 類似検索 - 分子機能 | ||||||
生物種 | Drosophila melanogaster (キイロショウジョウバエ) | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
データ登録者 | Avis, J.M. / Blankley, R.T. / Jennings, M.D. / Golovanov, A.P. | ||||||
引用 | ジャーナル: J.Biol.Chem. / 年: 2007 タイトル: Specificity and Autoregulation of Notch Binding by Tandem WW Domains in Suppressor of Deltex 著者: Jennings, M.D. / Blankley, R.T. / Baron, M. / Golovanov, A.P. / Avis, J.M. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2jmf.cif.gz | 356.8 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2jmf.ent.gz | 298.6 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2jmf.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 2jmf_validation.pdf.gz | 368.4 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 2jmf_full_validation.pdf.gz | 591.2 KB | 表示 | |
XML形式データ | 2jmf_validation.xml.gz | 16.4 KB | 表示 | |
CIF形式データ | 2jmf_validation.cif.gz | 30 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/jm/2jmf ftp://data.pdbj.org/pub/pdb/validation_reports/jm/2jmf | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 5928.586 Da / 分子数: 1 / 断片: WW4 domain, residues 515-557 / 由来タイプ: 組換発現 由来: (組換発現) Drosophila melanogaster (キイロショウジョウバエ) 遺伝子: Su dx / Plasmid details: cloned between EcoRI and XhoI / プラスミド: pGEX-6P-1 / 生物種 (発現宿主): Escherichia coli / 発現宿主: Escherichia coli BL21 (大腸菌) / 株 (発現宿主): BL21 参照: UniProt: Q9Y0H4, 合成酵素; C-N結合を形成; 酸-D-アミノ酸リガーゼ(ペプチド合成) |
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#2: タンパク質・ペプチド | 分子量: 2161.392 Da / 分子数: 1 / 断片: L/PPxY motif, residues 2318-2332 / 由来タイプ: 組換発現 由来: (組換発現) Drosophila melanogaster (キイロショウジョウバエ) 遺伝子: N / Plasmid details: cloned between BamHI and XhoI / プラスミド: pGEX-6P-1 / 生物種 (発現宿主): Escherichia coli / 発現宿主: Escherichia coli BL21 (大腸菌) / 株 (発現宿主): BL21 / 参照: UniProt: P07207, UniProt: Q32UW5*PLUS |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR実験 |
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-試料調製
詳細 |
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試料 |
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試料状態 | pH: 6.75 / 圧: ambient / 温度: 293 K |
-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M |
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放射波長 | 相対比: 1 |
NMRスペクトロメーター | タイプ: Bruker AMX / 製造業者: Bruker / モデル: AMX / 磁場強度: 600 MHz |
-解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 / 詳細: energy minimisation performed using AMBER | ||||||||||||||||||||||||||||
代表構造 | 選択基準: lowest energy | ||||||||||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: all calculated structures submitted 計算したコンフォーマーの数: 20 / 登録したコンフォーマーの数: 20 |