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Yorodumi- PDB-2jfz: Crystal structure of Helicobacter pylori glutamate racemase in co... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2jfz | ||||||
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Title | Crystal structure of Helicobacter pylori glutamate racemase in complex with D-Glutamate and an inhibitor | ||||||
Components | GLUTAMATE RACEMASE | ||||||
Keywords | ISOMERASE / CELL WALL / CELL SHAPE / GLUTAMATE RACEMASE / PEPTIDOGLYCAN SYNTHESIS / PEPTIDOGLYCAN BIOSYNTHESIS | ||||||
Function / homology | Function and homology information glutamate racemase / glutamate racemase activity / peptidoglycan biosynthetic process / cell wall organization / regulation of cell shape / identical protein binding Similarity search - Function | ||||||
Biological species | HELICOBACTER PYLORI (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.86 Å | ||||||
Authors | Lundqvist, T. | ||||||
Citation | Journal: Nature / Year: 2007 Title: Exploitation of Structural and Regulatory Diversity in Glutamate Racemases Authors: Lundqvist, T. / Fisher, S.L. / Kern, G. / Folmer, R.H.A. / Xue, Y. / Newton, D.T. / Keating, T.A. / Alm, R.A. / De Jonge, B.L.M. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR PROVIDED. | ||||||
Remark 700 | SHEET DETERMINATION METHOD: AUTHOR PROVIDED. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2jfz.cif.gz | 121.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2jfz.ent.gz | 94.7 KB | Display | PDB format |
PDBx/mmJSON format | 2jfz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2jfz_validation.pdf.gz | 1.2 MB | Display | wwPDB validaton report |
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Full document | 2jfz_full_validation.pdf.gz | 1.2 MB | Display | |
Data in XML | 2jfz_validation.xml.gz | 25.9 KB | Display | |
Data in CIF | 2jfz_validation.cif.gz | 37.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jf/2jfz ftp://data.pdbj.org/pub/pdb/validation_reports/jf/2jfz | HTTPS FTP |
-Related structure data
Related structure data | 2jfnC 2jfoC 2jfpC 2jfqC 2jfuC 2jfvC 2jfwC 2jfxSC 2jfyC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 28532.193 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HELICOBACTER PYLORI (bacteria) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: Q9ZLT0, glutamate racemase #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.1 Å3/Da / Density % sol: 44 % |
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Crystal grow | pH: 8.5 Details: PROTEIN FORMULATED AT 10 MG/ML WITH 200 MM AMMONIUM ACETATE PH 7.4, 5 MM D-L GLUTAMATE, 1 MM TCEP AND CRYSTALLISED WITH 100 MM TRIS PH 8, 200 MM AMMONIUM SULFATE, 25% PEG 3350 AND 20% GLYCEROL |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU300 / Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.86→15 Å / Num. obs: 40333 / % possible obs: 93.6 % / Observed criterion σ(I): 2 / Redundancy: 3.1 % / Rmerge(I) obs: 0.07 / Net I/σ(I): 9.1 |
Reflection shell | Resolution: 1.86→1.96 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.42 / Mean I/σ(I) obs: 1.7 / % possible all: 86.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2JFX Resolution: 1.86→15 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Bsol: 33.2255 Å2 / ksol: 0.365438 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21.6 Å2
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Refinement step | Cycle: LAST / Resolution: 1.86→15 Å
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Refine LS restraints |
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