double-stranded DNA 3'-5' DNA exonuclease activity / exodeoxyribonuclease III / endonuclease activity / DNA repair / DNA binding / metal ion binding 類似検索 - 分子機能
Exodeoxyribonuclease III-like / AP endonuclease 1, binding site / AP endonucleases family 1 signature 1. / AP endonuclease 1 / AP endonucleases family 1 profile. / Deoxyribonuclease I; Chain A / Endonuclease/exonuclease/phosphatase / Endonuclease/exonuclease/phosphatase / Endonuclease/Exonuclease/phosphatase family / Endonuclease/exonuclease/phosphatase superfamily ...Exodeoxyribonuclease III-like / AP endonuclease 1, binding site / AP endonucleases family 1 signature 1. / AP endonuclease 1 / AP endonucleases family 1 profile. / Deoxyribonuclease I; Chain A / Endonuclease/exonuclease/phosphatase / Endonuclease/exonuclease/phosphatase / Endonuclease/Exonuclease/phosphatase family / Endonuclease/exonuclease/phosphatase superfamily / 4-Layer Sandwich / Alpha Beta 類似検索 - ドメイン・相同性
DIHYDROGENPHOSPHATE ION / ACETATE ION / Exodeoxyribonuclease III 類似検索 - 構成要素
pH: 6.5 詳細: PROTEIN CONCENTRATION 8.7MG/ML BUFFER: 10 MM TRIS, PH 7.0, 100 MM NACL . WELL SOLUTION: 100 MM NA CACODYLATE PH 6 200 MM NA ACETATE, 15 % PEG 8000. THE CRYSTALLISATION PLATES USED WERE ...詳細: PROTEIN CONCENTRATION 8.7MG/ML BUFFER: 10 MM TRIS, PH 7.0, 100 MM NACL . WELL SOLUTION: 100 MM NA CACODYLATE PH 6 200 MM NA ACETATE, 15 % PEG 8000. THE CRYSTALLISATION PLATES USED WERE CORNING ROUND BOTTOM 96 WELL PLATES. THE CRYSTALLISATION DROPS CONSISTED OF 1UL PROTEIN AND 1UL WELL SOLUTION.
解像度: 1.9→19.96 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.938 / SU B: 2.953 / SU ML: 0.088 / 交差検証法: THROUGHOUT / ESU R: 0.155 / ESU R Free: 0.142 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.FIVE RESIDUES HAVE UNUSUAL PHI PSI VALUES. THESE ARE RESIDUES SER 10, THR 61, ASN 63, GLU 110 AND HIS 167. THESE RESIDUES FIT THE ELECTRON ...詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.FIVE RESIDUES HAVE UNUSUAL PHI PSI VALUES. THESE ARE RESIDUES SER 10, THR 61, ASN 63, GLU 110 AND HIS 167. THESE RESIDUES FIT THE ELECTRON DENSITY WELL IN OMIT MAPS AND THEY ARE THOUGHT TO BE THE RESULT OF USUAL BACKBONE CONFORMATIONS. RESIDUES CYS 156 AND CYS 168 FORM A PARTIAL DISULPHIDE BRIDGE.
Rfactor
反射数
%反射
Selection details
Rfree
0.202
982
5.18 %
RAMDOM
Rwork
0.152
-
-
-
obs
-
19112
100 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK BULK SOLVENT