CELL INVASION / SHIGELLA FLEXNERI / TYPE III SECRETION / IPAD / T3SS / PLASMID / INVASIN / VIRULENCE
機能・相同性
機能・相同性情報
effector-mediated activation of programmed cell death in host / extracellular region 類似検索 - 分子機能
IpaD-like / IpaD-like / Type III secretion systems tip complex components / BipD-like superfamily / Type III secretion systems tip complex components / Up-down Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
ジャーナル: J Biol Chem / 年: 2007 タイトル: Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD. 著者: Steven Johnson / Pietro Roversi / Marianela Espina / Andrew Olive / Janet E Deane / Susan Birket / Terry Field / William D Picking / Ariel J Blocker / Edouard E Galyov / Wendy L Picking / Susan M Lea / 要旨: Bacteria expressing type III secretion systems (T3SS) have been responsible for the deaths of millions worldwide, acting as key virulence elements in diseases ranging from plague to typhoid fever. ...Bacteria expressing type III secretion systems (T3SS) have been responsible for the deaths of millions worldwide, acting as key virulence elements in diseases ranging from plague to typhoid fever. The T3SS is composed of a basal body, which traverses both bacterial membranes, and an external needle through which effector proteins are secreted. We report multiple crystal structures of two proteins that sit at the tip of the needle and are essential for virulence: IpaD from Shigella flexneri and BipD from Burkholderia pseudomallei. The structures reveal that the N-terminal domains of the molecules are intramolecular chaperones that prevent premature oligomerization, as well as sharing structural homology with proteins involved in eukaryotic actin rearrangement. Crystal packing has allowed us to construct a model for the tip complex that is supported by mutations designed using the structure.
THIS IS THE N102H VARIANT (FROM PLASMID PMYSH6000 AND PLASMID PCP301). THE HIS IN THE SEQUENCE IS A ...THIS IS THE N102H VARIANT (FROM PLASMID PMYSH6000 AND PLASMID PCP301). THE HIS IN THE SEQUENCE IS A NATURALLY OCCURRING VARIANT
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.48 Å3/Da / 溶媒含有率: 50.48 %
結晶化
pH: 8.5 詳細: 25-28 % (W/V) PEG 4000, 0.1 M TRIS-HCL, PH 8.5, 0.2 M MGCL2