+Open data
-Basic information
Entry | Database: PDB / ID: 2iux | ||||||
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Title | Human tACE mutant g1234 | ||||||
Components | ANGIOTENSIN-CONVERTING ENZYME | ||||||
Keywords | HYDROLASE / GLYCOSIDASE / POLYMORPHISM / GLYCOPROTEIN / METAL-BINDING / ALTERNATIVE SPLICING / METALLOPROTEASE / PHOSPHORYLATION / AC / ZINC / MEMBRANE / CHLORIDE / PROTEASE / TYPE-I MEMBRANE-ANCHORED PROTEIN / CARBOXYPEPTIDASE / PEPTIDYL DIPEPTIDASE / TRANSMEMBRANE | ||||||
Function / homology | Function and homology information mononuclear cell proliferation / cell proliferation in bone marrow / bradykinin receptor binding / regulation of angiotensin metabolic process / exopeptidase activity / tripeptidyl-peptidase activity / substance P catabolic process / peptidyl-dipeptidase A / regulation of renal output by angiotensin / negative regulation of calcium ion import ...mononuclear cell proliferation / cell proliferation in bone marrow / bradykinin receptor binding / regulation of angiotensin metabolic process / exopeptidase activity / tripeptidyl-peptidase activity / substance P catabolic process / peptidyl-dipeptidase A / regulation of renal output by angiotensin / negative regulation of calcium ion import / positive regulation of peptidyl-cysteine S-nitrosylation / response to laminar fluid shear stress / positive regulation of systemic arterial blood pressure / negative regulation of gap junction assembly / metallodipeptidase activity / cellular response to aldosterone / hormone catabolic process / bradykinin catabolic process / angiogenesis involved in coronary vascular morphogenesis / response to thyroid hormone / negative regulation of glucose import / vasoconstriction / antigen processing and presentation of peptide antigen via MHC class I / neutrophil mediated immunity / hormone metabolic process / regulation of smooth muscle cell migration / regulation of hematopoietic stem cell proliferation / mitogen-activated protein kinase binding / embryo development ending in birth or egg hatching / chloride ion binding / positive regulation of neurogenesis / mitogen-activated protein kinase kinase binding / arachidonic acid secretion / post-transcriptional regulation of gene expression / eating behavior / heterocyclic compound binding / peptide catabolic process / heart contraction / lung alveolus development / response to dexamethasone / regulation of heart rate by cardiac conduction / regulation of systemic arterial blood pressure by renin-angiotensin / regulation of vasoconstriction / peptidyl-dipeptidase activity / hematopoietic stem cell differentiation / angiotensin maturation / blood vessel remodeling / amyloid-beta metabolic process / animal organ regeneration / Metabolism of Angiotensinogen to Angiotensins / carboxypeptidase activity / positive regulation of vasoconstriction / sperm midpiece / blood vessel diameter maintenance / response to nutrient levels / basal plasma membrane / kidney development / female pregnancy / angiotensin-activated signaling pathway / brush border membrane / cellular response to glucose stimulus / regulation of synaptic plasticity / metalloendopeptidase activity / regulation of blood pressure / metallopeptidase activity / male gonad development / actin binding / peptidase activity / spermatogenesis / endopeptidase activity / response to lipopolysaccharide / lysosome / calmodulin binding / response to hypoxia / endosome / response to xenobiotic stimulus / positive regulation of apoptotic process / external side of plasma membrane / negative regulation of gene expression / proteolysis / extracellular space / extracellular exosome / zinc ion binding / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / OTHER / Resolution: 2.8 Å | ||||||
Authors | Watermeyer, J.M. / Swell, B.T. / Natesh, R. / Corradi, H.R. / Acharya, K.R. / Sturrock, E.D. | ||||||
Citation | Journal: Biochemistry / Year: 2006 Title: Structure of Testis Ace Glycosylation Mutants and Evidence for Conserved Domain Movement. Authors: Watermeyer, J.M. / Sewell, B.T. / Schwager, S.L. / Natesh, R. / Corradi, H.R. / Acharya, K.R. / Sturrock, E.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2iux.cif.gz | 124.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2iux.ent.gz | 99.3 KB | Display | PDB format |
PDBx/mmJSON format | 2iux.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/iu/2iux ftp://data.pdbj.org/pub/pdb/validation_reports/iu/2iux | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein / Sugars , 2 types, 3 molecules A
#1: Protein | Mass: 68325.242 Da / Num. of mol.: 1 / Fragment: RESIDUES 68-658 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Cell line (production host): CHINESE HAMSTER OVARY CELLS / Production host: CRICETULUS GRISEUS (Chinese hamster) References: UniProt: P22966, UniProt: P12821*PLUS, Hydrolases; Glycosylases; Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds, peptidyl-dipeptidase A |
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#2: Sugar |
-Non-polymers , 5 types, 38 molecules
#3: Chemical | ChemComp-ACT / | ||||
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#4: Chemical | ChemComp-ZN / | ||||
#5: Chemical | #6: Chemical | ChemComp-NXA / | #7: Water | ChemComp-HOH / | |
-Details
Compound details | ENGINEERED |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.32 % |
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Crystal grow | pH: 4.7 Details: 0.1M SODIUM ACETATE PH4.7 15% PEG 4000, 10UM ZINC SULPHATE, pH 4.70 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX14.1 / Wavelength: 1.488 |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.488 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→50 Å / Num. obs: 13572 / % possible obs: 83.6 % / Observed criterion σ(I): 2 / Redundancy: 39 % / Rmerge(I) obs: 0.11 / Net I/σ(I): 7.7 |
Reflection shell | Resolution: 2.8→2.9 Å / Rmerge(I) obs: 0.34 / Mean I/σ(I) obs: 2.8 / % possible all: 80.4 |
-Processing
Software |
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Refinement | Method to determine structure: OTHER / Resolution: 2.8→50 Å / Data cutoff high absF: 10000 / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: MLF
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Solvent computation | Bsol: 14.8386 Å2 / ksol: 0.361842 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 20 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→50 Å
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Refine LS restraints |
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Xplor file |
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