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- PDB-2irt: INITIAL CRYSTALLOGRAPHIC ANALYSES OF A RECOMBINANT INTERLEUKIN-1 ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2irt | ||||||
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Title | INITIAL CRYSTALLOGRAPHIC ANALYSES OF A RECOMBINANT INTERLEUKIN-1 RECEPTOR ANTAGONIST PROTEIN | ||||||
![]() | INTERLEUKIN-1 RECEPTOR ANTAGONIST | ||||||
![]() | SIGNAL PROTEIN | ||||||
Function / homology | ![]() interleukin-1 type I receptor antagonist activity / interleukin-1 type II receptor antagonist activity / interleukin-1, type I receptor binding / interleukin-1 receptor antagonist activity / interleukin-1, type II receptor binding / negative regulation of interleukin-1-mediated signaling pathway / negative regulation of heterotypic cell-cell adhesion / interleukin-1 receptor binding / inflammatory response to antigenic stimulus / insulin secretion ...interleukin-1 type I receptor antagonist activity / interleukin-1 type II receptor antagonist activity / interleukin-1, type I receptor binding / interleukin-1 receptor antagonist activity / interleukin-1, type II receptor binding / negative regulation of interleukin-1-mediated signaling pathway / negative regulation of heterotypic cell-cell adhesion / interleukin-1 receptor binding / inflammatory response to antigenic stimulus / insulin secretion / Interleukin-10 signaling / response to glucocorticoid / cytokine activity / acute-phase response / lipid metabolic process / Interleukin-1 signaling / immune response / centrosome / extracellular space / extracellular exosome / nucleoplasm / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Finzel, B.C. / Clancy, L.L. / Einspahr, H.M. | ||||||
![]() | ![]() Title: Initial crystallographic analysis of a recombinant human interleukin-1 receptor antagonist protein. Authors: Clancy, L.L. / Finzel, B.C. / Yem, A.W. / Deibel, M.R. / Strakalaitis, N.A. / Brunner, D.P. / Sweet, R.M. / Einspahr, H.M. #1: ![]() Title: Purification, Cloning, Expression and Biological Characterization of an Interleukin-1 Receptor Antagonist Protein Authors: Carter, D.B. / Deibel Junior, M.R. / Dunn, C.J. / Tomich, C.-S. / Laborde, A.L. / Slightom, J.L. / Berger, A.E. / Bienkowski, M.J. / Sun, F.F. / Mcewan, R.N. / Harris, P.K.W. / Yem, A.W. / ...Authors: Carter, D.B. / Deibel Junior, M.R. / Dunn, C.J. / Tomich, C.-S. / Laborde, A.L. / Slightom, J.L. / Berger, A.E. / Bienkowski, M.J. / Sun, F.F. / Mcewan, R.N. / Harris, P.K.W. / Yem, A.W. / Waszak, G.A. / Chosay, J.G. / Sieu, L.C. / Hardee, M.M. / Zurcher-Neely, H.A. / Reardon, I.M. / Heinrikson, R.L. / Truesdell, S.E. / Shelly, J.A. / Eessalu, T.T. / Taylor, B.M. / Tracey, D.E. | ||||||
History |
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Remark 700 | SHEET THE SHEETS PRESENTED AS *BLA* AND *BLB* ON SHEET RECORDS BELOW ARE ACTUALLY SIX-STRANDED BETA- ...SHEET THE SHEETS PRESENTED AS *BLA* AND *BLB* ON SHEET RECORDS BELOW ARE ACTUALLY SIX-STRANDED BETA-BARRELS. THIS IS REPRESENTED BY A SEVEN-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 19.1 KB | Display | ![]() |
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PDB format | ![]() | 9.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 17145.406 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.19 Å3/Da / Density % sol: 43.76 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 277 K / pH: 7 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 2.3 Å / Num. all: 14143 / Num. obs: 11831 / Num. measured all: 55341 / Rmerge(I) obs: 0.066 |
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Processing
Refinement | Resolution: 3.2→20 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 3.2→20 Å
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Refinement | *PLUS Rfactor Rwork: 0.44 | ||||||||||||
Solvent computation | *PLUS | ||||||||||||
Displacement parameters | *PLUS |