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基本情報
登録情報 | データベース: PDB / ID: 2ifg | |||||||||
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タイトル | Structure of the extracellular segment of human TRKA in complex with nerve growth factor | |||||||||
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![]() | TRANSFERASE / TRK / TRKA / NGF / NERVE GROWTH FACTOR / RECEPTOR-LIGAND COMPLEX | |||||||||
機能・相同性 | ![]() NFG and proNGF binds to p75NTR / Ceramide signalling / nerve growth factor receptor binding / NGF processing / behavioral response to formalin induced pain / neurotrophin p75 receptor binding / olfactory nerve development / response to hydrostatic pressure / TRKA activation by NGF / PLC-gamma1 signalling ...NFG and proNGF binds to p75NTR / Ceramide signalling / nerve growth factor receptor binding / NGF processing / behavioral response to formalin induced pain / neurotrophin p75 receptor binding / olfactory nerve development / response to hydrostatic pressure / TRKA activation by NGF / PLC-gamma1 signalling / programmed cell death involved in cell development / Signalling to STAT3 / p75NTR negatively regulates cell cycle via SC1 / neurotrophin receptor activity / mechanoreceptor differentiation / nerve growth factor receptor activity / metalloendopeptidase inhibitor activity / neurotrophin binding / Sertoli cell development / axonogenesis involved in innervation / GPI-linked ephrin receptor activity / nerve growth factor signaling pathway / nerve development / Retrograde neurotrophin signalling / Axonal growth stimulation / positive regulation of collateral sprouting / nerve growth factor binding / NGF-independant TRKA activation / NADE modulates death signalling / sympathetic nervous system development / Signalling to p38 via RIT and RIN / peripheral nervous system development / ARMS-mediated activation / positive regulation of Ras protein signal transduction / positive regulation of programmed cell death / positive regulation of synapse assembly / PI3K/AKT activation / regulation of neuron differentiation / Frs2-mediated activation / NRAGE signals death through JNK / regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / detection of temperature stimulus involved in sensory perception of pain / neurotrophin TRK receptor signaling pathway / extrinsic apoptotic signaling pathway via death domain receptors / response to axon injury / neuron development / Signalling to RAS / positive regulation of DNA binding / detection of mechanical stimulus involved in sensory perception of pain / response to electrical stimulus / peptidyl-tyrosine autophosphorylation / cell surface receptor protein tyrosine kinase signaling pathway / p75NTR recruits signalling complexes / positive regulation of neuron differentiation / NF-kB is activated and signals survival / NRIF signals cell death from the nucleus / transmembrane receptor protein tyrosine kinase activity / positive regulation of synaptic transmission, glutamatergic / response to nutrient levels / B cell differentiation / neuron projection morphogenesis / cellular response to nerve growth factor stimulus / positive regulation of GTPase activity / endosome lumen / axon guidance / growth factor activity / modulation of chemical synaptic transmission / receptor protein-tyrosine kinase / memory / kinase binding / positive regulation of neuron projection development / Golgi lumen / cellular response to nicotine / circadian rhythm / peptidyl-tyrosine phosphorylation / recycling endosome membrane / positive regulation of angiogenesis / neuron projection development / synaptic vesicle / late endosome / positive regulation of peptidyl-serine phosphorylation / late endosome membrane / positive regulation of NF-kappaB transcription factor activity / early endosome membrane / protein tyrosine kinase activity / neuron apoptotic process / negative regulation of neuron apoptotic process / protein autophosphorylation / learning or memory / positive regulation of ERK1 and ERK2 cascade / early endosome / receptor complex / endosome membrane / response to xenobiotic stimulus / positive regulation of protein phosphorylation / negative regulation of cell population proliferation / axon / protein phosphorylation / neuronal cell body / lipid binding 類似検索 - 分子機能 | |||||||||
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手法 | ![]() ![]() ![]() | |||||||||
![]() | He, X. / Garcia, K.C. | |||||||||
![]() | ![]() タイトル: Structural and mechanistic insights into nerve growth factor interactions with the TrkA and p75 receptors. 著者: Wehrman, T. / He, X. / Raab, B. / Dukipatti, A. / Blau, H. / Garcia, K.C. | |||||||||
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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PDBx/mmCIF形式 | ![]() | 191.2 KB | 表示 | ![]() |
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PDB形式 | ![]() | 154.1 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 3.3 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 3.4 MB | 表示 | |
XML形式データ | ![]() | 43.5 KB | 表示 | |
CIF形式データ | ![]() | 58.4 KB | 表示 | |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 37898.797 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() 発現宿主: ![]() ![]() 株 (発現宿主): TN5 / 参照: UniProt: P04629, receptor protein-tyrosine kinase #2: タンパク質 | 分子量: 13515.410 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() 発現宿主: ![]() ![]() 参照: UniProt: P01138 #3: 多糖 | #4: 多糖 | alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-4)-2-acetamido-2-deoxy- ...alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose #5: 多糖 | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 4.91 Å3/Da / 溶媒含有率: 75 % |
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結晶化 | pH: 7.5 詳細: PEG, GLYCINE, PH 7.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K, pH 7.50 |
-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: ADSC QUANTUM 4 / 検出器: CCD / 日付: 2004年4月15日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1 Å / 相対比: 1 |
反射 | 解像度: 3.4→50 Å / Num. obs: 26668 / % possible obs: 99 % / Observed criterion σ(I): 0 |
反射 シェル | 解像度: 3.4→3.5 Å / % possible all: 97 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: 1WWW 解像度: 3.4→12 Å / 交差検証法: THROUGHOUT / σ(F): 0 / 立体化学のターゲット値: Engh & Huber
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精密化ステップ | サイクル: LAST / 解像度: 3.4→12 Å
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LS精密化 シェル | 解像度: 3.4→3.5 Å / Rfactor Rfree: 0.391 / Rfactor Rwork: 0.37 |