Formation of Senescence-Associated Heterochromatin Foci (SAHF) / sexual sporulation resulting in formation of a cellular spore / H3 histone acetyltransferase complex / global genome nucleotide-excision repair / RNA polymerase I upstream activating factor complex / Condensation of Prophase Chromosomes / SIRT1 negatively regulates rRNA expression / Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 / Assembly of the ORC complex at the origin of replication / acetyltransferase activator activity ...Formation of Senescence-Associated Heterochromatin Foci (SAHF) / sexual sporulation resulting in formation of a cellular spore / H3 histone acetyltransferase complex / global genome nucleotide-excision repair / RNA polymerase I upstream activating factor complex / Condensation of Prophase Chromosomes / SIRT1 negatively regulates rRNA expression / Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 / Assembly of the ORC complex at the origin of replication / acetyltransferase activator activity / DNA replication-dependent chromatin assembly / nucleosome disassembly / replication fork protection complex / Oxidative Stress Induced Senescence / silent mating-type cassette heterochromatin formation / positive regulation of transcription by RNA polymerase I / RNA Polymerase I Promoter Escape / nucleolar large rRNA transcription by RNA polymerase I / Estrogen-dependent gene expression / rRNA transcription / negative regulation of DNA damage checkpoint / subtelomeric heterochromatin formation / regulation of DNA repair / CENP-A containing nucleosome / positive regulation of transcription elongation by RNA polymerase II / regulation of protein phosphorylation / protein modification process / structural constituent of chromatin / nucleosome / nucleosome assembly / chromatin organization / histone binding / regulation of gene expression / chromosome, telomeric region / protein heterodimerization activity / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / DNA binding / nucleus / cytosol Similarity search - Function
SEQUENCE The yeast ASF1 gene was genetically modified in a pET28b (novagen) derivative plasmid that ...SEQUENCE The yeast ASF1 gene was genetically modified in a pET28b (novagen) derivative plasmid that contains a hepta-his tag, Maltose Binding Protein tag, and is tethered to Asf1 via a TEV protease cleavable linker. Ala A 1 is therefore a reminant of the TEV cleavage reaction. Ala A 156 and Ala A 157 are part of an Ala/Gly/Thr linker which connects Asf1 and H3, however, no density was seen for the other six residues in this linker region.
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