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Yorodumi- PDB-2i8f: Solution Conformation of the H47A Mutant of Pseudomonas stutzeri ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2i8f | ||||||
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Title | Solution Conformation of the H47A Mutant of Pseudomonas stutzeri ZoBell Ferrocytochrome c-551 | ||||||
Components | Cytochrome c-551 | ||||||
Keywords | ELECTRON TRANSPORT / helix-turn-helix / cytochrome | ||||||
Function / homology | Function and homology information electron transfer activity / periplasmic space / iron ion binding / heme binding Similarity search - Function | ||||||
Biological species | Pseudomonas stutzeri ZoBell (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model type details | minimized average | ||||||
Authors | Liang, Q. / Timkovich, R. | ||||||
Citation | Journal: Biophys.J. / Year: 2007 Title: Solution Conformation of the His-47 to Ala-47 Mutant of Pseudomonas stutzeri ZoBell Ferrocytochrome c-551. Authors: Liang, Q. / Miller, G.T. / Beeghley, C.A. / Graf, C.B. / Timkovich, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2i8f.cif.gz | 35.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2i8f.ent.gz | 27.5 KB | Display | PDB format |
PDBx/mmJSON format | 2i8f.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i8/2i8f ftp://data.pdbj.org/pub/pdb/validation_reports/i8/2i8f | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8505.787 Da / Num. of mol.: 1 / Mutation: H47A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas stutzeri ZoBell (bacteria) / Species: Pseudomonas stutzeri / Strain: ZoBell / Gene: nirM / Plasmid: pBR329 / Production host: Escherichia coli (E. coli) / Strain (production host): UT5600 / References: UniProt: P00101 |
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#2: Chemical | ChemComp-HEC / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.5 mM H47A c-551, 50 mM sodium phosphate buffer, 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 50 mM / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: The structures are based on a total of 1511 restraints, 1414 are NOE-derived distance constraints, 67 dihedral angle restraints, 30 distance restraints from hydrogen bonds. | ||||||||||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||||||||||
NMR ensemble | Conformers submitted total number: 1 |