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- PDB-2hyn: Complete ensemble of NMR structures of unphosphorylated human pho... -
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Basic information
Entry | Database: PDB / ID: 2hyn | ||||||
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Title | Complete ensemble of NMR structures of unphosphorylated human phospholamban pentamer | ||||||
![]() | Cardiac phospholamban | ||||||
![]() | MEMBRANE PROTEIN/SIGNALING PROTEIN / symmetric homo-oligomer / pentamer / protein complex / LEU/ILE ZIPPER / SUPER COIL / CHANNEL / MEMBRANE PROTEIN-SIGNALING PROTEIN COMPLEX | ||||||
Function / homology | ![]() regulation of ATPase-coupled calcium transmembrane transporter activity / negative regulation of calcium ion binding / negative regulation of calcium ion import into sarcoplasmic reticulum / regulation of relaxation of cardiac muscle / negative regulation of ATPase-coupled calcium transmembrane transporter activity / adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process / regulation of the force of heart contraction by cardiac conduction / calcium ion-transporting ATPase complex / regulation of cardiac muscle cell membrane potential / acrosome assembly ...regulation of ATPase-coupled calcium transmembrane transporter activity / negative regulation of calcium ion binding / negative regulation of calcium ion import into sarcoplasmic reticulum / regulation of relaxation of cardiac muscle / negative regulation of ATPase-coupled calcium transmembrane transporter activity / adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process / regulation of the force of heart contraction by cardiac conduction / calcium ion-transporting ATPase complex / regulation of cardiac muscle cell membrane potential / acrosome assembly / negative regulation of calcium ion transmembrane transporter activity / negative regulation of calcium ion import / negative regulation of catalytic activity / regulation of the force of heart contraction / ATPase inhibitor activity / negative regulation of ATP-dependent activity / cardiac muscle tissue development / regulation of cardiac muscle cell contraction / blood circulation / enzyme inhibitor activity / relaxation of cardiac muscle / regulation of heart contraction / negative regulation of heart rate / muscle cell cellular homeostasis / regulation of calcium ion transport / Ion transport by P-type ATPases / negative regulation of calcium ion transport / regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion / Ion homeostasis / Notch signaling pathway / regulation of cytosolic calcium ion concentration / sarcoplasmic reticulum membrane / sarcoplasmic reticulum / mitochondrial membrane / intracellular calcium ion homeostasis / ATPase binding / endoplasmic reticulum membrane / perinuclear region of cytoplasm / endoplasmic reticulum / protein homodimerization activity / mitochondrion / identical protein binding / membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / Complete SCS search, Energy-minimization | ||||||
![]() | Potluri, S. / Yan, A.K. / Chou, J.J. / Donald, B.R. / Bailey-Kellogg, C. | ||||||
![]() | ![]() Title: Structure determination of symmetric homo-oligomers by a complete search of symmetry configuration space, using NMR restraints and van der Waals packing. Authors: Potluri, S. / Yan, A.K. / Chou, J.J. / Donald, B.R. / Bailey-Kellogg, C. #1: ![]() Title: The structure of phospholamban pentamer reveals a channel-like architecture in membranes Authors: Oxenoid, K. / Chou, J.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 15.4 MB | Display | ![]() |
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PDB format | ![]() | 13.1 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 6114.576 Da / Num. of mol.: 5 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: TROSY-HNCA, HN(CA)CB, HN(CA)CO, MEASUREMENT OF 1H-15N, 13C'- 13CA, AND 13C'-15N RDCS USING 3D HNCO BASED EXPERIMENTS, H(CCO)NH, C(CO)NH, 15N-EDITED NOESY, 13C-EDITED NOESY, 13C- HSQC FOR METHYL ...Type: TROSY-![]() ![]() |
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Sample preparation
Details | Contents: 0.2 MM PHOSPHOLAMBAN PENTAMER, U-15N, 13C, 85% 2H, 25 MM SODIUM PHOSPHATE BUFFER, 50 MM BME, 200 MM DPC, 95% H2O, 5% D2O;0.2 MM PHOSPHOLAMBAN PENTAMER, U-15N, 13C, 85% 2H, 25 MM SODIUM ...Contents: 0.2 MM PHOSPHOLAMBAN PENTAMER, U-15N, 13C, 85% 2H, 25 MM SODIUM PHOSPHATE BUFFER, 50 MM BME, 200 MM DPC, 95% H2O, 5% D2O;0.2 MM PHOSPHOLAMBAN PENTAMER, U-15N, 13C, 85% 2H, 25 MM SODIUM PHOSPHATE BUFFER, 50 MM BME, 200 MM DPC, 4% STRETCHED POLYACRYLAMIDE GEL,95% H2O, 5% D2O;0.2 MM PHOSPHOLAMBAN PENTAMER, U-15N,13C, 25 MM SODIUM PHOSPHATE BUFFER, 50 MM BME, 200 MM 2H-DPC, 95% H2O, 5% D2O;0.2 MM PHOSPHOLAMBAN PENTAMER, 10% 13C-LABELED, 25 MM SODIUM PHOSPHATE BUFFER, 50 MM BME, 200 MM DPC, 95% H2O, 5% D2O;0.2 MM PHOSPHOLAMBAN PENTAMER, 50% U-15N,13C AND 50% UNLABELED, 25MM SODIUM PHOSPHATE BUFFER, 50 MM BME, 200 MM 2H-DPC, 95% H2O, 5% D2O;0.2 MM PHOSPHOLAMBAN PENTAMER, 50% U-15N, U-2H AND 50% UNLABELED,25MM SODIUM PHOSPHATE BUFFER, 50 MM BME, 200 MM 2H-DPC, 95% H2O, 5% D2O Solvent system: 95% H2O/5% D2O |
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Sample conditions | Ionic strength: 25 mM SODIUM PHOSPHATE / pH: 6 / Pressure: AMBIENT / Temperature: 303 K |
-NMR measurement
Radiation wavelength | Relative weight: 1 |
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NMR spectrometer | Type: Bruker Adavnce, DMX / Manufacturer: Bruker / Model: Adavnce, DMX / Field strength: 500 MHz |
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Processing
NMR software |
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Refinement | Method: Complete SCS search, Energy-minimization / Software ordinal: 1 Details: 1. MONOMER STRUCTURE: DETERMINED WITH SIMULATED ANNEALING PROTOCOL THAT REFINES ALL INTRAMONOMER NOES, SIDECHAIN J-COUPLINGS, AND BACKBONE RDCS. 2. PENTAMER STRUCTURE: DETERMINED BY OUR ...Details: 1. MONOMER STRUCTURE: DETERMINED WITH SIMULATED ANNEALING PROTOCOL THAT REFINES ALL INTRAMONOMER NOES, SIDECHAIN J-COUPLINGS, AND BACKBONE RDCS. 2. PENTAMER STRUCTURE: DETERMINED BY OUR COMPLETE SCS APPROACH. TRADITIONAL NMR PROTOCOLS FOR SYMMETRIC HOMO-OLIGOMERS (USED FOR STRUCTURES OF PDB ID 1ZLL) ARE BASED ON SIMULATED ANNEALING TECHNIQUES AND COULD GET TRAPPED IN LOCAL MINIMA. OUR COMPLETE SCS SEARCH APPROACH AVOIDS BIAS AND RETURNS ALL CONSISTENT, WELL-PACKED STRUCTURES TO WITHIN A USER-DEFINED SIMILARITY LEVEL. ENERGY-MINIMIZATION IS USED ONLY AT A LATER STAGE AFTER THE ENSEMBLE OF STRUCTURES THAT SATISFY THE DATA HAVE BEEN IDENTIFIED, HENCE ENSURING THAT NO CONSISTENT WELL-PACKED CONFORMATION IS MISSED. | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: All calculated structures were submitted. Our method guarantees that the ensemble represents all structures that satisfy the data and have good vdW packing within a user- ...Conformer selection criteria: All calculated structures were submitted. Our method guarantees that the ensemble represents all structures that satisfy the data and have good vdW packing within a user-defined similarity level (chosen as 1 A). Conformers calculated total number: 184 / Conformers submitted total number: 184 |