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Open data
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Basic information
Entry | Database: PDB / ID: 1nq2 | ||||||
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Title | Two RTH Mutants with Impaired Hormone Binding | ||||||
![]() | Thyroid hormone receptor beta-1 | ||||||
![]() | HORMONE/GROWTH FACTOR RECEPTOR / ALPHA HELICAL / LIGAND BINDING DOMAIN / HORMONE-GROWTH FACTOR RECEPTOR COMPLEX | ||||||
Function / homology | ![]() retinal cone cell apoptotic process / negative regulation of female receptivity / female courtship behavior / retinal cone cell development / thyroid hormone receptor signaling pathway / positive regulation of thyroid hormone receptor signaling pathway / cellular response to thyroid hormone stimulus / regulation of heart contraction / type I pneumocyte differentiation / thyroid hormone binding ...retinal cone cell apoptotic process / negative regulation of female receptivity / female courtship behavior / retinal cone cell development / thyroid hormone receptor signaling pathway / positive regulation of thyroid hormone receptor signaling pathway / cellular response to thyroid hormone stimulus / regulation of heart contraction / type I pneumocyte differentiation / thyroid hormone binding / retinoic acid receptor signaling pathway / sensory perception of sound / SUMOylation of intracellular receptors / mRNA transcription by RNA polymerase II / chromatin DNA binding / transcription coactivator binding / Nuclear Receptor transcription pathway / RNA polymerase II transcription regulator complex / nuclear receptor activity / sequence-specific double-stranded DNA binding / cell differentiation / nuclear body / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / DNA-templated transcription / chromatin / enzyme binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / nucleoplasm / nucleus Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Huber, B.R. / Sandler, B. / West, B.L. / Cunha-Lima, S.T. / Nguyen, H.T. / Apriletti, J.W. / Baxter, J.D. / Fletterick, R.J. | ||||||
![]() | ![]() Title: Two resistance to thyroid hormone mutants with impaired hormone binding Authors: Huber, B.R. / Sandler, B. / West, B.L. / Cunha-Lima, S.T. / Nguyen, H.T. / Apriletti, J.W. / Baxter, J.D. / Fletterick, R.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 69.7 KB | Display | ![]() |
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PDB format | ![]() | 49.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 434.4 KB | Display | ![]() |
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Full document | ![]() | 444.6 KB | Display | |
Data in XML | ![]() | 8.3 KB | Display | |
Data in CIF | ![]() | 13 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 29755.361 Da / Num. of mol.: 1 / Fragment: LIGAND BINDING DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
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#2: Chemical | ChemComp-NA / | ||
#3: Chemical | ChemComp-4HY / [ | ||
#4: Chemical | ChemComp-ARS / #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.03 Å3/Da / Density % sol: 59.39 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.2 Details: 700 mM sodium acetate, 200mM sodium succinate 100mM sodium cacodylate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 8 / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE Details: Pt-coated, fused silica, vertical focusmirror, Cyclindrically bent triangular Si asymmetric cut, horizontal focus monochromator |
Radiation | Monochromator: Cyclindrically bent triangular Si asymmetric cut, horizontal focus monochromator Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→100 Å / Num. all: 96808 / Num. obs: 96581 / % possible obs: 99.76 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 25.122 % / Biso Wilson estimate: 40.79 Å2 / Rmerge(I) obs: 0.065 / Rsym value: 0.052 / Net I/σ(I): 105.6 |
Reflection | *PLUS Lowest resolution: 100 Å / Num. obs: 13799 / % possible obs: 93.3 % / Num. measured all: 54104 / Rmerge(I) obs: 0.055 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: THYROID HORMONE RECEPTOR BETA WITH TRIAC BOUND (unpublished) Resolution: 2.4→30 Å / Isotropic thermal model: ISOTROPIC / Cross valid method: THROUGHOUT / σ(F): 1 / σ(I): 1 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 6.881 Å2 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→30 Å
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Refinement | *PLUS Num. reflection obs: 12403 / % reflection Rfree: 10 % / Rfactor Rfree: 0.259 / Rfactor Rwork: 0.222 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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