+Open data
-Basic information
Entry | Database: PDB / ID: 2hlc | ||||||
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Title | HL COLLAGENASE STRUCTURE AT 1.7A RESOLUTION | ||||||
Components | COLLAGENASE | ||||||
Keywords | SERINE PROTEASE / HYDROLASE / COLLAGEN DEGRADATION | ||||||
Function / homology | Function and homology information hypodermin C / collagen catabolic process / serine-type endopeptidase activity / proteolysis / extracellular region Similarity search - Function | ||||||
Biological species | Hypoderma lineatum (early cattle grub) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / RIGID BODY / Resolution: 1.7 Å | ||||||
Authors | Broutin, I. / Merigeau, K. / Arnoux, B. / Ducruix, A. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1996 Title: 1.8 A structure of Hypoderma lineatum collagenase: a member of the serine proteinase family. Authors: Broutin, I. / Arnoux, B. / Riche, C. / Lecroisey, A. / Keil, B. / Pascard, C. / Ducruix, A. #1: Journal: To be Published Title: Collagenase-Collagenase Interactions, a Descriptor for the Mapping of the Binding Site Authors: Broutin, I. / Merigeau, K. / Arnoux, B. / Ducruix, A. #2: Journal: To be Published Title: Crystallographic Analyses of Lysozyme and Collagenase Space Grown Crystals Versus Ground Controls Authors: Broutin, I. / Ries-Kautt, M. / Ducruix, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2hlc.cif.gz | 101.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2hlc.ent.gz | 81.9 KB | Display | PDB format |
PDBx/mmJSON format | 2hlc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hl/2hlc ftp://data.pdbj.org/pub/pdb/validation_reports/hl/2hlc | HTTPS FTP |
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-Related structure data
Related structure data | 1hylSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 25247.189 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Hypoderma lineatum (early cattle grub) / Organ: STOMACH References: UniProt: P08897, Hydrolases; Acting on peptide bonds (peptidases); Serine endopeptidases #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.54 Å3/Da / Density % sol: 50 % | ||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion - hanging drop in microgravity / pH: 7 Details: PROTEIN CONCENTRATION WAS 10MG/ML. IT WAS CRYSTALLIZED IN 1.4M AMMONIUM SULFATE BY HANGING DROP METHOD IN APCF REACTORS UNDER MICROGRAVITY (IML2 MISSION)., pH 7.0, vapor diffusion - hanging drop in microgravity | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 291 K / Method: microdialysis / Details: or vapor diffusion | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 280 K |
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Diffraction source | Source: SYNCHROTRON / Site: LURE / Beamline: DW32 / Wavelength: 0.9375 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Nov 1, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9375 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→15 Å / Num. obs: 54909 / % possible obs: 96.3 % / Observed criterion σ(I): 0 / Redundancy: 4.4 % / Biso Wilson estimate: 23.18 Å2 / Rsym value: 0.047 / Net I/σ(I): 11.4 |
Reflection shell | Resolution: 1.7→1.74 Å / Redundancy: 3.8 % / Mean I/σ(I) obs: 1.6 / Rsym value: 0.478 / % possible all: 99.6 |
Reflection | *PLUS Rmerge(I) obs: 0.047 |
Reflection shell | *PLUS % possible obs: 99.6 % / Rmerge(I) obs: 0.478 |
-Processing
Software |
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Refinement | Method to determine structure: RIGID BODY Starting model: PDB ENTRY 1HYL Resolution: 1.7→8 Å / Cross valid method: THROUGHOUT
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Displacement parameters | Biso mean: 22.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.23 Å / Luzzati d res low obs: 8 Å / Luzzati sigma a obs: 0.21 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→8 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.7→1.78 Å / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.3428 |