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- PDB-2hid: REFINED NMR STRUCTURE OF PHOSPHOCARRIER HISTIDINE CONTAINING PROT... -

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Basic information

Entry
Database: PDB / ID: 2hid
TitleREFINED NMR STRUCTURE OF PHOSPHOCARRIER HISTIDINE CONTAINING PROTEIN FROM BACILLUS SUBTILIS
ComponentsHISTIDINE CONTAINING PROTEIN
KeywordsPHOSPHOTRANSFERASE / HISTIDINE CONTAINING PROTEIN / PTS
Function / homology
Function and homology information


regulation of carbohydrate utilization / phosphoenolpyruvate-dependent sugar phosphotransferase system / cytoplasm
Similarity search - Function
Phosphotransferase system, HPr histidine phosphorylation site / PTS HPR domain histidine phosphorylation site signature. / Phosphotransferase system, HPr serine phosphorylation site / HPr-like / Phosphocarrier protein HPr-like / HPr-like superfamily / : / PTS HPr component phosphorylation site / PTS HPR domain profile. / Histidine-containing Protein; Chain: A; ...Phosphotransferase system, HPr histidine phosphorylation site / PTS HPR domain histidine phosphorylation site signature. / Phosphotransferase system, HPr serine phosphorylation site / HPr-like / Phosphocarrier protein HPr-like / HPr-like superfamily / : / PTS HPr component phosphorylation site / PTS HPR domain profile. / Histidine-containing Protein; Chain: A; / PTS HPR domain serine phosphorylation site signature. / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Phosphocarrier protein HPr
Similarity search - Component
Biological speciesBacillus subtilis (bacteria)
MethodSOLUTION NMR
AuthorsJones, B.E. / Rajagopal, P. / Klevit, R.E.
Citation
Journal: Protein Sci. / Year: 1997
Title: Phosphorylation on histidine is accompanied by localized structural changes in the phosphocarrier protein, HPr from Bacillus subtilis.
Authors: Jones, B.E. / Rajagopal, P. / Klevit, R.E.
#1: Journal: Protein Sci. / Year: 1992
Title: Solution Structure of the Phosphocarrier Protein Hpr from Bacillus Subtilis by Two-Dimensional NMR Spectroscopy
Authors: Wittekind, M. / Rajagopal, P. / Branchini, B.R. / Reizer, J. / Saier Junior, M.H. / Klevit, R.E.
#2: Journal: Biochemistry / Year: 1990
Title: Sequence-Specific 1H NMR Resonance Assignments of Bacillus Subtilis Hpr: Use of Spectra Obtained from Mutants to Resolve Spectral Overlap
Authors: Wittekind, M. / Reizer, J. / Klevit, R.E.
#3: Journal: Biochemistry / Year: 1989
Title: Common Structural Changes Accompany the Functional Inactivation of Hpr by Seryl Phosphorylation or by Serine to Aspartate Substitution
Authors: Wittekind, M. / Reizer, J. / Deutscher, J. / Saier, M.H. / Klevit, R.E.
History
DepositionMay 17, 1996Processing site: BNL
SupersessionNov 8, 1996ID: 1HID
Revision 1.0Nov 8, 1996Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Nov 3, 2021Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site / _struct_ref_seq_dif.details
Revision 1.4May 29, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: HISTIDINE CONTAINING PROTEIN


Theoretical massNumber of molelcules
Total (without water)9,0351
Polymers9,0351
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)25 / -
Representative

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Components

#1: Protein HISTIDINE CONTAINING PROTEIN / HPR


Mass: 9035.073 Da / Num. of mol.: 1 / Mutation: M51V
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Bacillus subtilis (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: P08877

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR detailsText: 50 MM PHOSPHATE BUFFER

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Sample preparation

Sample conditionspH: 6.9 / Temperature: 303 K
Crystal grow
*PLUS
Method: other / Details: NMR

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Processing

Software
NameVersionClassification
X-PLOR3.1model building
X-PLOR3.1refinement
X-PLOR3.1phasing
NMR softwareName: X-PLOR / Version: 3.1 / Developer: BRUNGER / Classification: refinement
NMR ensembleConformers submitted total number: 25

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