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- PDB-2het: Non-myristoylated bovine recoverin (truncated at C-terminus) with... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2het | ||||||
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Title | Non-myristoylated bovine recoverin (truncated at C-terminus) with calcium bound to EF-hand 3 | ||||||
![]() | Recoverin | ||||||
![]() | METAL BINDING PROTEIN / Recoverin / EF-hand / helix-loop-helix / calcium binding | ||||||
Function / homology | ![]() Inactivation, recovery and regulation of the phototransduction cascade / regulation of calcium ion transport / phototransduction / photoreceptor outer segment / visual perception / photoreceptor inner segment / perikaryon / calcium ion binding / membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Weiergraber, O.H. / Granzin, J. | ||||||
![]() | ![]() Title: Tuning of a neuronal calcium sensor. Authors: Weiergraber, O.H. / Senin, I.I. / Zernii, E.Y. / Churumova, V.A. / Kovaleva, N.A. / Nazipova, A.A. / Permyakov, S.E. / Permyakov, E.A. / Philippov, P.P. / Granzin, J. / Koch, K.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 141.5 KB | Display | ![]() |
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PDB format | ![]() | 111.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1omrS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 21748.322 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-CA / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.45 Å3/Da / Density % sol: 49.71 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 2.4 M sodium malonate, 2 mM calcium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Feb 25, 2006 / Details: Monochromators, mirrors |
Radiation | Monochromator: Diamond, germanium / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 3→70 Å / Num. all: 14380 / Num. obs: 14380 / % possible obs: 84.8 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 2.4 % / Biso Wilson estimate: 89.4 Å2 / Rmerge(I) obs: 0.044 / Rsym value: 0.044 / Net I/σ(I): 11.3 |
Reflection shell | Resolution: 3→3.16 Å / Redundancy: 2.2 % / Rmerge(I) obs: 0.234 / Mean I/σ(I) obs: 1.7 / Num. unique all: 2072 / Rsym value: 0.234 / % possible all: 84.1 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1OMR Resolution: 3→70 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 77.4 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3→70 Å
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Refine LS restraints |
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