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- PDB-1omr: non-myristoylated wild-type bovine recoverin with calcium bound t... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1omr | ||||||
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Title | non-myristoylated wild-type bovine recoverin with calcium bound to EF-hand 3 | ||||||
![]() | recoverin | ||||||
![]() | METAL BINDING PROTEIN / EF-hand / helix-loop-helix | ||||||
Function / homology | ![]() Inactivation, recovery and regulation of the phototransduction cascade / regulation of calcium ion transport / phototransduction / photoreceptor outer segment / visual perception / photoreceptor inner segment / perikaryon / calcium ion binding / membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Weiergraber, O.H. / Granzin, J. | ||||||
![]() | ![]() Title: Impact of N-terminal myristoylation on the Ca2+-dependent conformational transition in recoverin Authors: Weiergraber, O.H. / Senin, I.I. / Philippov, P.P. / Granzin, J. / Koch, K.-W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 55.4 KB | Display | ![]() |
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PDB format | ![]() | 39.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1omvC ![]() 1recS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 23235.209 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-CA / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.16 Å3/Da / Density % sol: 42.71 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 8 Details: ammonium sulfate, TRIS, calcium chloride, magnesium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Apr 21, 2002 / Details: mirrors |
Radiation | Monochromator: mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→48.39 Å / Num. all: 33042 / Num. obs: 33042 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.8 % / Biso Wilson estimate: 19.5 Å2 / Rmerge(I) obs: 0.058 / Rsym value: 0.058 / Net I/σ(I): 5.2 |
Reflection shell | Resolution: 1.5→1.54 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.36 / Mean I/σ(I) obs: 2 / Num. unique all: 2444 / Rsym value: 0.36 / % possible all: 100 |
Reflection | *PLUS Highest resolution: 1.5 Å / Rmerge(I) obs: 0.068 |
Reflection shell | *PLUS % possible obs: 100 % / Rmerge(I) obs: 0.422 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 1REC Resolution: 1.5→48.39 Å / Isotropic thermal model: anisotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 22.3 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.5→48.39 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.5→1.52 Å / Rfactor Rfree error: 0.058
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Refinement | *PLUS Rfactor Rfree: 0.25 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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