[English] 日本語
Yorodumi- PDB-1omr: non-myristoylated wild-type bovine recoverin with calcium bound t... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1omr | ||||||
---|---|---|---|---|---|---|---|
Title | non-myristoylated wild-type bovine recoverin with calcium bound to EF-hand 3 | ||||||
Components | recoverin | ||||||
Keywords | METAL BINDING PROTEIN / EF-hand / helix-loop-helix | ||||||
Function / homology | Function and homology information Inactivation, recovery and regulation of the phototransduction cascade / regulation of calcium ion transport / phototransduction / photoreceptor outer segment / photoreceptor inner segment / visual perception / perikaryon / calcium ion binding / membrane / cytosol Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.5 Å | ||||||
Authors | Weiergraber, O.H. / Granzin, J. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2003 Title: Impact of N-terminal myristoylation on the Ca2+-dependent conformational transition in recoverin Authors: Weiergraber, O.H. / Senin, I.I. / Philippov, P.P. / Granzin, J. / Koch, K.-W. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1omr.cif.gz | 55.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1omr.ent.gz | 39.3 KB | Display | PDB format |
PDBx/mmJSON format | 1omr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/om/1omr ftp://data.pdbj.org/pub/pdb/validation_reports/om/1omr | HTTPS FTP |
---|
-Related structure data
Related structure data | 1omvC 1recS C: citing same article (ref.) S: Starting model for refinement |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 23235.209 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bos taurus (cattle) / Gene: RCV1 / Plasmid: pET11d / Production host: Escherichia coli (E. coli) / Strain (production host): rec/BL21 / References: UniProt: P21457 |
---|---|
#2: Chemical | ChemComp-CA / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.16 Å3/Da / Density % sol: 42.71 % | |||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 8 Details: ammonium sulfate, TRIS, calcium chloride, magnesium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-1 / Wavelength: 0.934 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Apr 21, 2002 / Details: mirrors |
Radiation | Monochromator: mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→48.39 Å / Num. all: 33042 / Num. obs: 33042 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.8 % / Biso Wilson estimate: 19.5 Å2 / Rmerge(I) obs: 0.058 / Rsym value: 0.058 / Net I/σ(I): 5.2 |
Reflection shell | Resolution: 1.5→1.54 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.36 / Mean I/σ(I) obs: 2 / Num. unique all: 2444 / Rsym value: 0.36 / % possible all: 100 |
Reflection | *PLUS Highest resolution: 1.5 Å / Rmerge(I) obs: 0.068 |
Reflection shell | *PLUS % possible obs: 100 % / Rmerge(I) obs: 0.422 |
-Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 1REC Resolution: 1.5→48.39 Å / Isotropic thermal model: anisotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
| |||||||||||||||||||||||||
Displacement parameters | Biso mean: 22.3 Å2
| |||||||||||||||||||||||||
Refine analyze |
| |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.5→48.39 Å
| |||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||
LS refinement shell | Resolution: 1.5→1.52 Å / Rfactor Rfree error: 0.058
| |||||||||||||||||||||||||
Refinement | *PLUS Rfactor Rfree: 0.25 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
|