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Yorodumi- PDB-2had: CRYSTAL STRUCTURE OF HALOALKANE DEHALOGENASE: AN ENZYME TO DETOXI... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2had | ||||||
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Title | CRYSTAL STRUCTURE OF HALOALKANE DEHALOGENASE: AN ENZYME TO DETOXIFY HALOGENATED ALKANES | ||||||
Components | HALOALKANE DEHALOGENASE | ||||||
Keywords | DEHALOGENASE | ||||||
Function / homology | Function and homology information 1,2-dichloroethane catabolic process / haloalkane dehalogenase / haloalkane dehalogenase activity / response to toxic substance Similarity search - Function | ||||||
Biological species | Xanthobacter autotrophicus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.9 Å | ||||||
Authors | Verschueren, K.H.G. / Franken, S.M. / Dijkstra, B.W. | ||||||
Citation | Journal: EMBO J. / Year: 1991 Title: Crystal structure of haloalkane dehalogenase: an enzyme to detoxify halogenated alkanes. Authors: Franken, S.M. / Rozeboom, H.J. / Kalk, K.H. / Dijkstra, B.W. #1: Journal: J.Mol.Biol. / Year: 1988 Title: Crystallization of Haloalkane Dehalogenase from Xanthobacter Autotrophicus Gj10 Authors: Rozeboom, H.J. / Kingma, J. / Janssen, D.B. / Dijkstra, B.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2had.cif.gz | 77.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2had.ent.gz | 58.6 KB | Display | PDB format |
PDBx/mmJSON format | 2had.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ha/2had ftp://data.pdbj.org/pub/pdb/validation_reports/ha/2had | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUES PRO 57 AND PRO 168 ARE CIS PROLINES. |
-Components
#1: Protein | Mass: 35175.797 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Xanthobacter autotrophicus (bacteria) / References: UniProt: P22643 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.03 Å3/Da / Density % sol: 39.39 % | |||||||||||||||
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Crystal | *PLUS Density % sol: 39 % | |||||||||||||||
Crystal grow | *PLUS pH: 6.2 / Method: vapor diffusion, hanging drop | |||||||||||||||
Components of the solutions | *PLUS
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-Processing
Software | Name: TNT / Classification: refinement | ||||||||||||
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Refinement | Resolution: 1.9→7 Å / Rfactor obs: 0.1684 | ||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→7 Å
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Refinement | *PLUS Num. reflection obs: 20296 | ||||||||||||
Solvent computation | *PLUS | ||||||||||||
Displacement parameters | *PLUS |