[English] 日本語
Yorodumi- PDB-2ha1: Complex of the first and second type III domains of human Fibrone... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2ha1 | ||||||
---|---|---|---|---|---|---|---|
Title | Complex of the first and second type III domains of human Fibronectin in solution | ||||||
Components | Fibronectin | ||||||
Keywords | CELL ADHESION / STRUCTURAL PROTEIN / Beta sandwich / Protein-Protein complex / Rigid body docking | ||||||
Function / homology | Function and homology information negative regulation of monocyte activation / calcium-independent cell-matrix adhesion / negative regulation of transforming growth factor beta production / Fibronectin matrix formation / Extracellular matrix organization / positive regulation of substrate-dependent cell migration, cell attachment to substrate / neural crest cell migration involved in autonomic nervous system development / peptidase activator activity / fibrinogen complex / peptide cross-linking ...negative regulation of monocyte activation / calcium-independent cell-matrix adhesion / negative regulation of transforming growth factor beta production / Fibronectin matrix formation / Extracellular matrix organization / positive regulation of substrate-dependent cell migration, cell attachment to substrate / neural crest cell migration involved in autonomic nervous system development / peptidase activator activity / fibrinogen complex / peptide cross-linking / integrin activation / ALK mutants bind TKIs / cell-substrate junction assembly / biological process involved in interaction with symbiont / proteoglycan binding / Molecules associated with elastic fibres / MET activates PTK2 signaling / extracellular matrix structural constituent / Syndecan interactions / p130Cas linkage to MAPK signaling for integrins / endodermal cell differentiation / GRB2:SOS provides linkage to MAPK signaling for Integrins / endoplasmic reticulum-Golgi intermediate compartment / Non-integrin membrane-ECM interactions / basement membrane / ECM proteoglycans / Integrin cell surface interactions / positive regulation of axon extension / collagen binding / Degradation of the extracellular matrix / Integrin signaling / substrate adhesion-dependent cell spreading / regulation of ERK1 and ERK2 cascade / cell-matrix adhesion / extracellular matrix / platelet alpha granule lumen / acute-phase response / integrin-mediated signaling pathway / Cell surface interactions at the vascular wall / Post-translational protein phosphorylation / regulation of protein phosphorylation / Signaling by high-kinase activity BRAF mutants / wound healing / MAP2K and MAPK activation / response to wounding / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / GPER1 signaling / Signaling downstream of RAS mutants / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / Signaling by BRAF and RAF1 fusions / positive regulation of fibroblast proliferation / Signaling by ALK fusions and activated point mutants / integrin binding / Platelet degranulation / nervous system development / heparin binding / heart development / regulation of cell shape / angiogenesis / Interleukin-4 and Interleukin-13 signaling / collagen-containing extracellular matrix / protease binding / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / blood microparticle / cell adhesion / apical plasma membrane / endoplasmic reticulum lumen / signaling receptor binding / positive regulation of cell population proliferation / positive regulation of gene expression / extracellular space / extracellular exosome / extracellular region / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / Rigid body docking with torsional flexibility at the interfacial amino acid sidechains | ||||||
Authors | Vakonakis, I. / Campbell, I.D. | ||||||
Citation | Journal: Embo J. / Year: 2007 Title: Interdomain association in fibronectin: insight into cryptic sites and fibrillogenesis. Authors: Vakonakis, I. / Staunton, D. / Rooney, L.M. / Campbell, I.D. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2ha1.cif.gz | 2.5 MB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2ha1.ent.gz | 2.2 MB | Display | PDB format |
PDBx/mmJSON format | 2ha1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2ha1_validation.pdf.gz | 347.4 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 2ha1_full_validation.pdf.gz | 705.6 KB | Display | |
Data in XML | 2ha1_validation.xml.gz | 210.6 KB | Display | |
Data in CIF | 2ha1_validation.cif.gz | 248.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ha/2ha1 ftp://data.pdbj.org/pub/pdb/validation_reports/ha/2ha1 | HTTPS FTP |
-Related structure data
Related structure data | 2h41C 2h45C C: citing same article (ref.) |
---|---|
Similar structure data | |
Other databases |
|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 22113.336 Da / Num. of mol.: 1 Fragment: Fibronectin first and second type III domain pair (1F3-2F3) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: FN1 / Plasmid: pGEX-6P-2 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)RP / References: UniProt: P02751 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample conditions | Ionic strength: 150mM NaCl, 20mM Na2HPO3 / pH: 7.0 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
NMR spectrometer | Type: Home-built Home build / Manufacturer: Home-built / Model: Home build / Field strength: 750 MHz |
---|
-Processing
NMR software |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: Rigid body docking with torsional flexibility at the interfacial amino acid sidechains Software ordinal: 1 Details: The structure was calculated by rigid body docking using ambiguous interdomain NOE-like restraints derived from chemical shift mapping of the interface and residual dipolar couplings of the ...Details: The structure was calculated by rigid body docking using ambiguous interdomain NOE-like restraints derived from chemical shift mapping of the interface and residual dipolar couplings of the complex. 7 residues from each domain were used for the ambiguous NOEs as well as 72 RDCs total. The refinement protocol was similar to that of Clore & Schwieters, JACS 125, 2902 | ||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: Structures forming a single cluster after imposing a 50% lowest energy cutoff Conformers calculated total number: 100 / Conformers submitted total number: 39 |