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- PDB-2h31: Crystal structure of human PAICS, a bifunctional carboxylase and ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2h31 | ||||||
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Title | Crystal structure of human PAICS, a bifunctional carboxylase and synthetase in purine biosynthesis | ||||||
![]() | Multifunctional protein ADE2 | ||||||
![]() | LIGASE / LYASE / alpha-beta-alpha | ||||||
Function / homology | ![]() phosphoribosylaminoimidazole carboxylase / : / phosphoribosylaminoimidazole carboxylase activity / phosphoribosylaminoimidazolesuccinocarboxamide synthase / phosphoribosylaminoimidazolesuccinocarboxamide synthase activity / purine nucleobase biosynthetic process / 'de novo' XMP biosynthetic process / Purine ribonucleoside monophosphate biosynthesis / 'de novo' AMP biosynthetic process / GMP biosynthetic process ...phosphoribosylaminoimidazole carboxylase / : / phosphoribosylaminoimidazole carboxylase activity / phosphoribosylaminoimidazolesuccinocarboxamide synthase / phosphoribosylaminoimidazolesuccinocarboxamide synthase activity / purine nucleobase biosynthetic process / 'de novo' XMP biosynthetic process / Purine ribonucleoside monophosphate biosynthesis / 'de novo' AMP biosynthetic process / GMP biosynthetic process / 'de novo' IMP biosynthetic process / cadherin binding / extracellular exosome / ATP binding / identical protein binding / membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Li, S.-X. / Tong, Y.-P. / Xie, X.-C. / Li, S.-G. / Bi, R.-C. | ||||||
![]() | ![]() Title: Octameric structure of the human bifunctional enzyme PAICS in purine biosynthesis. Authors: Li, S.X. / Tong, Y.P. / Xie, X.C. / Wang, Q.H. / Zhou, H.N. / Han, Y. / Zhang, Z.Y. / Gao, W. / Li, S.G. / Zhang, X.C. / Bi, R.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 84 KB | Display | ![]() |
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PDB format | ![]() | 67.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 437.3 KB | Display | ![]() |
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Full document | ![]() | 456.2 KB | Display | |
Data in XML | ![]() | 18.2 KB | Display | |
Data in CIF | ![]() | 23.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Details | The biological assembly is a octamer generated from the monomer in the asymmetric unit by the operations: -x,-y,z and -y,x,z and y,-x,z and -x,y,-z and x,-y,-z and y,x,-z and -y,-x,-z. |
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Components
#1: Protein | Mass: 47463.367 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P22234, phosphoribosylaminoimidazolesuccinocarboxamide synthase, phosphoribosylaminoimidazole carboxylase |
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#2: Chemical | ChemComp-CO2 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.85 Å3/Da / Density % sol: 56.86 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 1.0M succinic acid pH 7.0, 0.1M hepes pH 7.0, 0.5%(w/v) PEG1500, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 28, 2005 | ||||||||||||
Radiation | Monochromator: SGM-TRAIN / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.8→50 Å / Num. all: 14136 / Num. obs: 14136 / % possible obs: 100 % / Observed criterion σ(F): 3 / Observed criterion σ(I): 3 | ||||||||||||
Reflection shell | Resolution: 2.8→2.9 Å / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 2.8→50 Å
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Refine LS restraints |
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