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Open data
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Basic information
Entry | Database: PDB / ID: 2g0k | ||||||
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Title | Solution Structure of Neocarzinostatin Apo-Protein | ||||||
![]() | Neocarzinostatin | ||||||
![]() | ANTIBIOTIC / BETA SANDWICH / IGG FOLD | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
![]() | Muskett, F.W. / Stoneman, R.G. / Caddick, S. / Woolfson, D.N. | ||||||
![]() | ![]() Title: Synthetic Ligands for Apo-Neocarzinostatin Authors: Caddick, S. / Muskett, F.W. / Stoneman, R.G. / Woolfson, D.N. #1: ![]() Title: Solution structure of a novel chromoprotein derived from apo-neocarzinostatin and a synthetic chromophore. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.3 MB | Display | ![]() |
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PDB format | ![]() | 1.2 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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NMR ensembles |
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Components
#1: Protein | Mass: 12227.273 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: ncsA / Plasmid: PCANTABB5 / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 1mM apo-neocarzinostatin U-15N,13C; 25 MM SODIUM PHOSPHATE, 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 50mM / pH: 5 / Pressure: ambient / Temperature: 308 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 47 |