+Open data
-Basic information
Entry | Database: PDB / ID: 2fgh | ||||||
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Title | ATP bound gelsolin | ||||||
Components | gelsolin | ||||||
Keywords | CONTRACTILE PROTEIN / STRUCTURAL PROTEIN / gelsolin / ATP | ||||||
Function / homology | Function and homology information actin filament severing / barbed-end actin filament capping / actin polymerization or depolymerization / cell projection assembly / cilium assembly / phosphatidylinositol-4,5-bisphosphate binding / central nervous system development / actin filament binding / actin cytoskeleton / extracellular space ...actin filament severing / barbed-end actin filament capping / actin polymerization or depolymerization / cell projection assembly / cilium assembly / phosphatidylinositol-4,5-bisphosphate binding / central nervous system development / actin filament binding / actin cytoskeleton / extracellular space / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Equus caballus (horse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||
Authors | Ma, Q. / Robinson, R.C. / Burtnick, L.D. / Urosev, D. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2006 Title: The structure of gelsolin bound to ATP Authors: Urosev, D. / Ma, Q. / Tan, A.L.C. / Robinson, R.C. / Burtnick, L.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2fgh.cif.gz | 260.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2fgh.ent.gz | 220.4 KB | Display | PDB format |
PDBx/mmJSON format | 2fgh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fg/2fgh ftp://data.pdbj.org/pub/pdb/validation_reports/fg/2fgh | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / Refine code: 1
NCS ensembles :
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Details | THE BIOLOGICAL ASSEMBLY IS FROM A SINGLE POLYPEPTIDE CHAIN FOLDED INTO SIX STRUCTURALLY SIMIALAR DOMAINS, S1 THROUGH S6. |
-Components
#1: Protein | Mass: 80921.867 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Equus caballus (horse) / References: UniProt: Q28372 #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.24 Å3/Da / Density % sol: 62.04 % |
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Crystal grow | Method: vapor diffusion / pH: 8 Details: crystals were grown from drops containing a 1:1 ratio of 34% saturated ammonium sulfate solution, 100mM Tris-HCL reservoir solution, pH 8.0, VAPOR DIFFUSION |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: MAX II / Beamline: I711 / Wavelength: 0.957 Å |
Detector | Detector: AREA DETECTOR |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.957 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→30 Å / Num. all: 52123 / Num. obs: 52123 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.2 % |
Reflection shell | Resolution: 2.8→2.9 Å / % possible all: 97 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.8→30 Å / Cor.coef. Fo:Fc: 0.91 / Cor.coef. Fo:Fc free: 0.889 / SU B: 15.402 / SU ML: 0.293 / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / ESU R: 0.596 / ESU R Free: 0.364 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 57.155 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→30 Å
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Refine LS restraints |
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Refine LS restraints NCS | Dom-ID: 1 / Auth asym-ID: A / Refine-ID: X-RAY DIFFRACTION / Type: TIGHT POSITIONAL / Weight position: 0.05
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LS refinement shell | Resolution: 2.8→2.872 Å / Total num. of bins used: 20
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