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Yorodumi- PDB-2fci: Structural basis for the requirement of two phosphotyrosines in s... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2fci | |||||||||
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Title | Structural basis for the requirement of two phosphotyrosines in signaling mediated by Syk tyrosine kinase | |||||||||
Components |
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Keywords | HYDROLASE / SH2 domain / phosphopeptide / Syk kinase / PLCgamma / PLCC | |||||||||
Function / homology | Function and homology information calcium-dependent phospholipase C activity / phosphoinositide phospholipase C / phospholipid catabolic process / phosphatidylinositol metabolic process / phosphatidylinositol phospholipase C activity / COP9 signalosome / positive regulation of epithelial cell migration / phosphatidylinositol-mediated signaling / cellular response to epidermal growth factor stimulus / cellular response to vascular endothelial growth factor stimulus ...calcium-dependent phospholipase C activity / phosphoinositide phospholipase C / phospholipid catabolic process / phosphatidylinositol metabolic process / phosphatidylinositol phospholipase C activity / COP9 signalosome / positive regulation of epithelial cell migration / phosphatidylinositol-mediated signaling / cellular response to epidermal growth factor stimulus / cellular response to vascular endothelial growth factor stimulus / release of sequestered calcium ion into cytosol / ruffle / guanyl-nucleotide exchange factor activity / epidermal growth factor receptor signaling pathway / non-specific protein-tyrosine kinase / ruffle membrane / non-membrane spanning protein tyrosine kinase activity / lamellipodium / adaptive immune response / in utero embryonic development / intracellular signal transduction / calcium ion binding / ATP binding / plasma membrane / cytoplasm Similarity search - Function | |||||||||
Biological species | Bos taurus (cattle) | |||||||||
Method | SOLUTION NMR | |||||||||
Model type details | minimized average | |||||||||
Authors | Groesch, T.D. / Zhou, F. / Mattila, S. / Geahlen, R.L. / Post, C.B. | |||||||||
Citation | Journal: J.Mol.Biol. / Year: 2006 Title: Structural basis for the requirement of two phosphotyrosine residues in signaling mediated by syk tyrosine kinase Authors: Groesch, T.D. / Zhou, F. / Mattila, S. / Geahlen, R.L. / Post, C.B. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2fci.cif.gz | 613.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2fci.ent.gz | 503.8 KB | Display | PDB format |
PDBx/mmJSON format | 2fci.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2fci_validation.pdf.gz | 376.8 KB | Display | wwPDB validaton report |
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Full document | 2fci_full_validation.pdf.gz | 669.8 KB | Display | |
Data in XML | 2fci_validation.xml.gz | 73.6 KB | Display | |
Data in CIF | 2fci_validation.cif.gz | 89.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fc/2fci ftp://data.pdbj.org/pub/pdb/validation_reports/fc/2fci | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1699.510 Da / Num. of mol.: 1 / Fragment: Phosphopeptide from Syk kinase / Source method: obtained synthetically / Details: Peptide Synthesis / References: UniProt: Q32PK0*PLUS |
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#2: Protein | Mass: 12275.924 Da / Num. of mol.: 1 / Fragment: C-terminal SH2 domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bos taurus (cattle) / Gene: PLCG1 / Plasmid: pET11d / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / Variant (production host): BL21(DE3) References: UniProt: P08487, phosphoinositide phospholipase C |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1 mM SH2 domain, 1 mM peptide, 20 mM MES, 100 mM NaCl, 3 mM Tris(2-carboxyethyl)-phosphine |
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Sample conditions | Ionic strength: 100mM / pH: 6 / Pressure: 1 atm / Temperature: 278 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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NMR representative | Selection criteria: minimized average structure | |||||||||
NMR ensemble | Conformer selection criteria: Lack of NOE violations & correct main chain geometry Conformers calculated total number: 50 / Conformers submitted total number: 16 |