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Open data
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Basic information
Entry | Database: PDB / ID: 2fa2 | ||||||
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Title | Crystal structure of Fus3 without a peptide from Ste5 | ||||||
![]() | Mitogen-activated protein kinase FUS3 | ||||||
![]() | TRANSFERASE / MAP kinase | ||||||
Function / homology | ![]() MAPK3 (ERK1) activation / phospho-PLA2 pathway / RAF-independent MAPK1/3 activation / MAPK1 (ERK2) activation / Frs2-mediated activation / ERK/MAPK targets / Signalling to ERK5 / ERKs are inactivated / Senescence-Associated Secretory Phenotype (SASP) / NCAM signaling for neurite out-growth ...MAPK3 (ERK1) activation / phospho-PLA2 pathway / RAF-independent MAPK1/3 activation / MAPK1 (ERK2) activation / Frs2-mediated activation / ERK/MAPK targets / Signalling to ERK5 / ERKs are inactivated / Senescence-Associated Secretory Phenotype (SASP) / NCAM signaling for neurite out-growth / Recycling pathway of L1 / Signal transduction by L1 / RAF/MAP kinase cascade / MAP2K and MAPK activation / Negative feedback regulation of MAPK pathway / Negative regulation of MAPK pathway / Gastrin-CREB signalling pathway via PKC and MAPK / Estrogen-stimulated signaling through PRKCZ / Ca2+ pathway / pheromone response MAPK cascade / pheromone-dependent signal transduction involved in conjugation with cellular fusion / invasive growth in response to glucose limitation / Regulation of HSF1-mediated heat shock response / Activation of the AP-1 family of transcription factors / retrotransposon silencing / mating projection tip / negative regulation of MAPK cascade / MAPK6/MAPK4 signaling / MAP kinase activity / mitogen-activated protein kinase / Neutrophil degranulation / positive regulation of protein export from nucleus / cytoplasmic stress granule / periplasmic space / protein kinase activity / intracellular signal transduction / cell cycle / phosphorylation / cell division / protein serine kinase activity / protein serine/threonine kinase activity / mitochondrion / ATP binding / identical protein binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Bhattacharyya, R.P. / Remenyi, A. / Good, M.C. / Bashor, C.J. / Falick, A.M. / Lim, W.A. | ||||||
![]() | ![]() Title: The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway Authors: Bhattacharyya, R.P. / Remenyi, A. / Good, M.C. / Bashor, C.J. / Falick, A.M. / Lim, W.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 142.7 KB | Display | ![]() |
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PDB format | ![]() | 112.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 452.2 KB | Display | ![]() |
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Full document | ![]() | 476.5 KB | Display | |
Data in XML | ![]() | 27.8 KB | Display | |
Data in CIF | ![]() | 37.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2f49C ![]() 2f9gC ![]() 2b9fS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | The assymetric unit contains two biological units. The structure is a homodimer of MAP kinase Fus3. |
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Components
#1: Protein | Mass: 40809.047 Da / Num. of mol.: 2 / Mutation: T180V, Y182F Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: FUS3, DAC2 / Plasmid: pBH4-Fus3VF / Production host: ![]() ![]() #2: Chemical | ChemComp-SCN / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.59 Å3/Da / Density % sol: 52.52 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.1 Details: 18% PEG3350, 0.1M MES, 10% MPD, 0.2M KSCN, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Mar 13, 2004 |
Radiation | Monochromator: Double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1159 Å / Relative weight: 1 |
Reflection | Resolution: 2.85→50 Å / Num. all: 19082 / Num. obs: 19082 / % possible obs: 94.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.4 % / Rsym value: 0.074 / Net I/σ(I): 13.5 |
Reflection shell | Resolution: 2.85→2.95 Å / Mean I/σ(I) obs: 3.9 / Num. unique all: 1359 / Rsym value: 0.189 / % possible all: 68.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 2B9F Resolution: 2.85→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.85→20 Å
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Refine LS restraints |
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