+Open data
-Basic information
Entry | Database: PDB / ID: 2evn | |||||||||
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Title | NMR solution structures of At1g77540 | |||||||||
Components | Protein At1g77540 | |||||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / PSI structural genomics CESG At1g77540 Center for Eukaryotic structural genomics / Protein Structure Initiative | |||||||||
Function / homology | Function and homology information histone acetyltransferase activity / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / peroxisome Similarity search - Function | |||||||||
Biological species | Arabidopsis thaliana (thale cress) | |||||||||
Method | SOLUTION NMR / Torsion Angle Dynamics Cartesian Angle Dynamics nOe Based distance restraints | |||||||||
Authors | Tyler, R.C. / Singh, S. / Tonelli, M. / Min, M.S. / Markley, J.L. / Center for Eukaryotic Structural Genomics (CESG) | |||||||||
Citation | Journal: Biochemistry / Year: 2006 Title: Structure of Arabidopsis thaliana At1g77540 Protein, a Minimal Acetyltransferase from the COG2388 Family. Authors: Tyler, R.C. / Bitto, E. / Berndsen, C.E. / Bingman, C.A. / Singh, S. / Lee, M.S. / Wesenberg, G.E. / Denu, J.M. / Phillips Jr., G.N. / Markley, J.L. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2evn.cif.gz | 702.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2evn.ent.gz | 597.3 KB | Display | PDB format |
PDBx/mmJSON format | 2evn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2evn_validation.pdf.gz | 345.2 KB | Display | wwPDB validaton report |
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Full document | 2evn_full_validation.pdf.gz | 481.3 KB | Display | |
Data in XML | 2evn_validation.xml.gz | 39.4 KB | Display | |
Data in CIF | 2evn_validation.cif.gz | 64.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ev/2evn ftp://data.pdbj.org/pub/pdb/validation_reports/ev/2evn | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11752.433 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Plasmid: PQE / Production host: Escherichia coli (E. coli) / Strain (production host): B834 / References: UniProt: Q9CAQ2 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1mM At1g77540 U-15N,13C; 10mM KHPO4, 50mM KCl, 90%H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 50mM KCl / pH: 6 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: Torsion Angle Dynamics Cartesian Angle Dynamics nOe Based distance restraints Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |