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- PDB-2evn: NMR solution structures of At1g77540 -

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Basic information

Entry
Database: PDB / ID: 2evn
TitleNMR solution structures of At1g77540
ComponentsProtein At1g77540
KeywordsSTRUCTURAL GENOMICS / UNKNOWN FUNCTION / PSI structural genomics CESG At1g77540 Center for Eukaryotic structural genomics / Protein Structure Initiative
Function / homology
Function and homology information


histone acetyltransferase activity / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / peroxisome
Similarity search - Function
Gcn5-related N-acetyltransferase / Yjdj-type Gcn5-related N-acetyltransferase / GCN5-related N-acetyl-transferase / Yjdj-type Gcn5-related N-acetyltransferase (GNAT) domain profile. / Gcn5-related N-acetyltransferase (GNAT) / Acyl-CoA N-acyltransferase / Aminopeptidase / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
Acetyltransferase At1g77540
Similarity search - Component
Biological speciesArabidopsis thaliana (thale cress)
MethodSOLUTION NMR / Torsion Angle Dynamics Cartesian Angle Dynamics nOe Based distance restraints
AuthorsTyler, R.C. / Singh, S. / Tonelli, M. / Min, M.S. / Markley, J.L. / Center for Eukaryotic Structural Genomics (CESG)
CitationJournal: Biochemistry / Year: 2006
Title: Structure of Arabidopsis thaliana At1g77540 Protein, a Minimal Acetyltransferase from the COG2388 Family.
Authors: Tyler, R.C. / Bitto, E. / Berndsen, C.E. / Bingman, C.A. / Singh, S. / Lee, M.S. / Wesenberg, G.E. / Denu, J.M. / Phillips Jr., G.N. / Markley, J.L.
History
DepositionOct 31, 2005Deposition site: RCSB / Processing site: RCSB
SupersessionNov 15, 2005ID: 1XO4
Revision 1.0Nov 15, 2005Provider: repository / Type: Initial release
Revision 1.1May 1, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 9, 2022Group: Database references / Derived calculations
Category: database_2 / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.4May 22, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Protein At1g77540


Theoretical massNumber of molelcules
Total (without water)11,7521
Polymers11,7521
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 100structures with the lowest energy
RepresentativeModel #1lowest energy

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Components

#1: Protein Protein At1g77540


Mass: 11752.433 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Plasmid: PQE / Production host: Escherichia coli (E. coli) / Strain (production host): B834 / References: UniProt: Q9CAQ2

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1113D 13C-separated NOESY
1213D 15N-separated NOESY

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Sample preparation

DetailsContents: 1mM At1g77540 U-15N,13C; 10mM KHPO4, 50mM KCl, 90%H2O, 10% D2O
Solvent system: 90% H2O/10% D2O
Sample conditionsIonic strength: 50mM KCl / pH: 6 / Pressure: ambient / Temperature: 298 K

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NMR measurement

RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelengthRelative weight: 1
NMR spectrometerType: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz

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Processing

NMR software
NameVersionDeveloperClassification
CNS1.1Brungerstructure solution
CNS1.1Brungerrefinement
NMRPipe2.2Delaglioprocessing
RefinementMethod: Torsion Angle Dynamics Cartesian Angle Dynamics nOe Based distance restraints
Software ordinal: 1
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: structures with the lowest energy
Conformers calculated total number: 100 / Conformers submitted total number: 20

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