+Open data
-Basic information
Entry | Database: PDB / ID: 2crk | ||||||
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Title | MUSCLE CREATINE KINASE | ||||||
Components | PROTEIN (CREATINE KINASE) | ||||||
Keywords | TRANSFERASE / CREATINE KINASE | ||||||
Function / homology | Function and homology information creatine kinase / phosphocreatine biosynthetic process / creatine kinase activity / response to heat / phosphorylation / extracellular space / ATP binding Similarity search - Function | ||||||
Biological species | Oryctolagus cuniculus (rabbit) | ||||||
Method | X-RAY DIFFRACTION / MIR / Resolution: 2.35 Å | ||||||
Authors | Rao, J.K. / Bujacz, G. / Wlodawer, A. | ||||||
Citation | Journal: FEBS Lett. / Year: 1998 Title: Crystal structure of rabbit muscle creatine kinase. Authors: Rao, J.K. / Bujacz, G. / Wlodawer, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2crk.cif.gz | 95.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2crk.ent.gz | 72.5 KB | Display | PDB format |
PDBx/mmJSON format | 2crk.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cr/2crk ftp://data.pdbj.org/pub/pdb/validation_reports/cr/2crk | HTTPS FTP |
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-Related structure data
Related structure data | 1crkS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 43301.227 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Oryctolagus cuniculus (rabbit) / Organ: HEART / Tissue: MUSCLESkeletal muscle / References: UniProt: P00563, creatine kinase | ||
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#2: Chemical | ChemComp-SO4 / #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 4.2 Å3/Da / Density % sol: 68 % | |||||||||||||||||||||||||
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Crystal grow | pH: 7.2 Details: 55% TO 60% SATURATED AMMONIUM SULFATE, 2.5% PEG400 IN HEPES BUFFER AT PH 7.2, ABOUT 5MM/MG PROTEIN CONCENTRATION | |||||||||||||||||||||||||
Crystal | *PLUS | |||||||||||||||||||||||||
Crystal grow | *PLUS Method: standing drop | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 120 K |
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Diffraction source | Source: ROTATING ANODE / Type: ENRAF-NONIUS FR591 / Wavelength: 1.5418 |
Detector | Type: MAC Science DIP-2020 / Detector: IMAGE PLATE / Date: Oct 15, 1996 / Details: FRANKS DOUBLE MIRROR OPTICS |
Radiation | Monochromator: PT AND NI COATED GLASS MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.35→20 Å / Num. obs: 29042 / % possible obs: 96.6 % / Observed criterion σ(I): 1 / Redundancy: 9.4 % / Rsym value: 0.101 / Net I/σ(I): 16.45 |
Reflection shell | Resolution: 2.35→2.39 Å / Redundancy: 5.3 % / Mean I/σ(I) obs: 3.78 / Rsym value: 0.339 / % possible all: 89.2 |
Reflection | *PLUS Rmerge(I) obs: 0.101 |
-Processing
Software |
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Refinement | Method to determine structure: MIR Starting model: 1CRK Resolution: 2.35→7 Å / Cross valid method: THROUGHOUT / σ(F): 2
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Displacement parameters | Biso mean: 26.3 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.25 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.35→7 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.35→2.43 Å / Total num. of bins used: 10 /
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Xplor file | Serial no: 1 / Param file: PARAM19X.PRO / Topol file: TOPH19X.PRO | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 7 Å / σ(F): 2 / Num. reflection Rfree: 2943 / % reflection Rfree: 9 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 26.3 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.285 / % reflection Rfree: 9 % / Rfactor Rwork: 0.377 |