SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED.
Mass: 18.015 Da / Num. of mol.: 109 / Source method: isolated from a natural source / Formula: H2O
Compound details
ENGINEERED RESIDUE IN CHAIN A, LEU 1454 TO ILE ENGINEERED RESIDUE IN CHAIN A, ALA 1459 TO VAL ...ENGINEERED RESIDUE IN CHAIN A, LEU 1454 TO ILE ENGINEERED RESIDUE IN CHAIN A, ALA 1459 TO VAL ENGINEERED RESIDUE IN CHAIN A, LEU 1464 TO ALA ENGINEERED RESIDUE IN CHAIN A, VAL 1475 TO ILE ENGINEERED RESIDUE IN CHAIN A, TYR 1478 TO ILE ENGINEERED RESIDUE IN CHAIN A, ILE 1480 TO LEU ENGINEERED RESIDUE IN CHAIN A, PHE 1494 TO ILE ENGINEERED RESIDUE IN CHAIN A, VAL 1496 TO LEU ENGINEERED RESIDUE IN CHAIN A, ALA 1503 TO TYR ENGINEERED RESIDUE IN CHAIN A, VAL 1512 TO THR ENGINEERED RESIDUE IN CHAIN A, VAL 1518 TO LEU ENGINEERED RESIDUE IN CHAIN A, ALA 1520 TO SER ENGINEERED RESIDUE IN CHAIN A, ILE 1534 TO ALA ENGINEERED RESIDUE IN CHAIN A, TYR 1538 TO PHE ENGINEERED RESIDUE IN CHAIN B, LEU 1454 TO ILE ENGINEERED RESIDUE IN CHAIN B, ALA 1459 TO VAL ENGINEERED RESIDUE IN CHAIN B, LEU 1464 TO ALA ENGINEERED RESIDUE IN CHAIN B, VAL 1475 TO ILE ENGINEERED RESIDUE IN CHAIN B, TYR 1478 TO ILE ENGINEERED RESIDUE IN CHAIN B, ILE 1480 TO LEU ENGINEERED RESIDUE IN CHAIN B, PHE 1494 TO ILE ENGINEERED RESIDUE IN CHAIN B, VAL 1496 TO LEU ENGINEERED RESIDUE IN CHAIN B, ALA 1503 TO TYR ENGINEERED RESIDUE IN CHAIN B, VAL 1512 TO THR ENGINEERED RESIDUE IN CHAIN B, VAL 1518 TO LEU ENGINEERED RESIDUE IN CHAIN B, ALA 1520 TO SER ENGINEERED RESIDUE IN CHAIN B, ILE 1534 TO ALA ENGINEERED RESIDUE IN CHAIN B, TYR 1538 TO PHE
Sequence details
DELIBERATELY ENGINEERED MUTATION
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.32 Å3/Da / Density % sol: 47 %
Crystal grow
pH: 5.1 Details: 50% PEG-400, 0.1 M ACTEATE, 0.2 M LITHIUM SULPHATE, PH 5.1
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Data collection
Diffraction
Mean temperature: 100 K
Diffraction source
Source: ROTATING ANODE / Wavelength: 1.5418
Detector
Date: Feb 2, 2006
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1.5418 Å / Relative weight: 1
Reflection
Resolution: 2→19.55 Å / Num. obs: 12995 / % possible obs: 97.3 % / Observed criterion σ(I): 1 / Redundancy: 8.9 % / Rmerge(I) obs: 0.07 / Net I/σ(I): 23
Reflection shell
Resolution: 2→2.11 Å / Redundancy: 8.5 % / Rmerge(I) obs: 0.23 / Mean I/σ(I) obs: 8.9 / % possible all: 95.9
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