分子量: 42927.602 Da / 分子数: 2 / 変異: YES / 由来タイプ: 組換発現 詳細: GDP-BETA-L-GALACTOSE WAS REFINED USING GMP (DEFINED AS GDP, MODIFIED IN THE CIF FILE TO REMOVE THE SECOND PHOSPHATE GROUP) AND GALACTOSE-MONOPHOSPHATE (GA3), LINKING THE GDP O3A TO THE GA3 PB 由来: (組換発現) Arabidopsis thaliana (シロイヌナズナ) プラスミド: PHISTEV-GME-Y174F / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / Variant (発現宿主): Rosetta / 参照: UniProt: Q93VR3, GDP-mannose 3,5-epimerase
CATALYZES A REVERSIBLE EPIMERIZATION OF GDP-D-MANNOSE ENGINEERED RESIDUE IN CHAIN A, TYR 174 TO PHE ...CATALYZES A REVERSIBLE EPIMERIZATION OF GDP-D-MANNOSE ENGINEERED RESIDUE IN CHAIN A, TYR 174 TO PHE ENGINEERED RESIDUE IN CHAIN B, TYR 174 TO PHE
配列の詳細
THE FIRST TWO RESIDUES (GA) IN THE SPECIFIED SEQUENCE ARE A REMNANT FROM A CLEAVED HIS-TAG (AND ARE ...THE FIRST TWO RESIDUES (GA) IN THE SPECIFIED SEQUENCE ARE A REMNANT FROM A CLEAVED HIS-TAG (AND ARE NOT IN THE UNIPROT SEQUENCE), THE RESIDUE NUMBERING IN THE STRUCTURE USES THE THIRD RESIDUE (M) AS RESIDUE 1.
解像度: 1.4→21.61 Å / Cor.coef. Fo:Fc: 0.983 / Cor.coef. Fo:Fc free: 0.974 / SU B: 1.472 / SU ML: 0.027 / 交差検証法: THROUGHOUT / ESU R: 0.054 / ESU R Free: 0.049 / 立体化学のターゲット値: RESTRAINED 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. EACH MONOMER OF GME Y174F CONTAINS A NAD IN THE NUCLEOTIDE BINDING SITE, AND GDP-BETA-L-GALACTOSE IN THE NUCLEOTIDE SUGAR BINDING SITE.
Rfactor
反射数
%反射
Selection details
Rfree
0.14
6337
5.08 %
RANDOM
Rwork
0.103
-
-
-
obs
0.105
126798
96.5 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK BULK SOLVENT