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- PDB-2bw2: BofC from Bacillus subtilis -

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Basic information

Entry
Database: PDB / ID: 2bw2
TitleBofC from Bacillus subtilis
ComponentsBYPASS OF FORESPORE C
KeywordsSIGNALING PROTEIN / SPORULATION / BOFC / SIGMAK CHECKPOINT
Function / homology
Function and homology information


sporulation resulting in formation of a cellular spore
Similarity search - Function
Bypass-of-forespore C, N-terminal domain / Bypass-of-forespore C, C-terminal domain / Bypass of forespore C, C-terminal / Bypass-of-forespore C, N-terminal / Bypass of forespore C, C-terminal domain superfamily / Bypass-of-forespore C, N-terminal domain superfamily / BofC C-terminal domain / Bypass of Forespore C, N terminal / Ubiquitin-like (UB roll) / Alpha-Beta Plaits ...Bypass-of-forespore C, N-terminal domain / Bypass-of-forespore C, C-terminal domain / Bypass of forespore C, C-terminal / Bypass-of-forespore C, N-terminal / Bypass of forespore C, C-terminal domain superfamily / Bypass-of-forespore C, N-terminal domain superfamily / BofC C-terminal domain / Bypass of Forespore C, N terminal / Ubiquitin-like (UB roll) / Alpha-Beta Plaits / Roll / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Biological speciesBACILLUS SUBTILIS (bacteria)
MethodSOLUTION NMR / CANDID, CNS
AuthorsPatterson, H.M. / Brannigan, J.A. / Cutting, S.M. / Wilson, K.S. / Wilkinson, A.J. / Ab, E. / Diercks, T. / Folkers, G.E. / de Jong, R.N. / Truffault, V. / Kaptein, R.
CitationJournal: J. Biol. Chem. / Year: 2005
Title: The structure of bypass of forespore C, an intercompartmental signaling factor during sporulation in Bacillus.
Authors: Patterson, H.M. / Brannigan, J.A. / Cutting, S.M. / Wilson, K.S. / Wilkinson, A.J. / Ab, E. / Diercks, T. / de Jong, R.N. / Truffault, V. / Folkers, G.E. / Kaptein, R.
History
DepositionJul 8, 2005Deposition site: PDBE / Processing site: PDBE
Revision 1.0Sep 15, 2005Provider: repository / Type: Initial release
Revision 1.1May 8, 2011Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3May 9, 2018Group: Data collection / Database references
Category: citation / citation_author / pdbx_nmr_spectrometer
Item: _citation.journal_abbrev / _citation.page_last ..._citation.journal_abbrev / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.title / _citation_author.name / _pdbx_nmr_spectrometer.model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: BYPASS OF FORESPORE C


Theoretical massNumber of molelcules
Total (without water)16,1951
Polymers16,1951
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)25 / 100ENERGY
RepresentativeModel #1

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Components

#1: Protein BYPASS OF FORESPORE C


Mass: 16195.253 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) BACILLUS SUBTILIS (bacteria) / Strain: IG20 / Description: NCIMB11621 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / References: UniProt: O05391
Sequence detailsTHE PROTEIN PRECURSOR FOR BOFC HAS 170 RESIDUES. THE NMR STRUCTURE IS FOR MATURE BOFC, WITH THE N- ...THE PROTEIN PRECURSOR FOR BOFC HAS 170 RESIDUES. THE NMR STRUCTURE IS FOR MATURE BOFC, WITH THE N-TERMINAL 30 RESIDUE TRANSLOCATION SEQUENCE CLEAVED. THE NMR STRUCTURE REFERS TO RESIDUE NUMBERS FOR THE MATURE PROTEIN, IE RESIDUES A1-G140 (NOT A31-G170)

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR detailsText: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELED BOFC.

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Sample preparation

DetailsContents: 95% WATER/5% D20
Sample conditionsIonic strength: 20 mM / pH: 6.0 / Temperature: 298.0 K

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NMR measurement

NMR spectrometerType: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 700 MHz

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Processing

NMR software
NameDeveloperClassification
CNSBRUNGER,ADAMS,CLORE,DELANO,GROS, GROSSE- KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,READ, RICE,SIMONSON,WARRENrefinement
SPARKYstructure solution
RefinementMethod: CANDID, CNS / Software ordinal: 1
Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE.
NMR ensembleConformer selection criteria: ENERGY / Conformers calculated total number: 100 / Conformers submitted total number: 25

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