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Yorodumi- PDB-2bn5: P-Element Somatic Inhibitor Protein Complex with U1-70k proline-r... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2bn5 | ||||||
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Title | P-Element Somatic Inhibitor Protein Complex with U1-70k proline-rich peptide | ||||||
Components |
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Keywords | NUCLEAR PROTEIN / SPLICING / PROTEIN-PROTEIN INTERACTION / PROLINE-RICH PEPTIDE / INHIBITOR / COMPLEX / NUCLEAR PROTEIN-COMPLEX | ||||||
Function / homology | Function and homology information mRNA Splicing - Major Pathway / snRNA binding / regulation of mRNA splicing, via spliceosome / U1 snRNP / U2-type prespliceosome / oogenesis / precatalytic spliceosome / regulation of alternative mRNA splicing, via spliceosome / negative regulation of mRNA splicing, via spliceosome / regulation of RNA splicing ...mRNA Splicing - Major Pathway / snRNA binding / regulation of mRNA splicing, via spliceosome / U1 snRNP / U2-type prespliceosome / oogenesis / precatalytic spliceosome / regulation of alternative mRNA splicing, via spliceosome / negative regulation of mRNA splicing, via spliceosome / regulation of RNA splicing / U1 snRNA binding / catalytic step 2 spliceosome / spliceosomal complex / mRNA processing / mRNA splicing, via spliceosome / spermatogenesis / nuclear speck / mRNA binding / regulation of DNA-templated transcription / RNA binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | DROSOPHILA MELANOGASTER (fruit fly) | ||||||
Method | SOLUTION NMR / SIMULATED ANNEAING | ||||||
Authors | Ignjatovic, T. / Yang, J.-C. / Butler, P.J.G. / Neuhaus, D. / Nagai, K. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2005 Title: Structural Basis of the Interaction between P-Element Somatic Inhibitor and U1-70K Essential for the Alternative Splicing of P-Element Transposase. Authors: Ignjatovic, T. / Yang, J.-C. / Butler, J. / Neuhaus, D. / Nagai, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2bn5.cif.gz | 463 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2bn5.ent.gz | 388 KB | Display | PDB format |
PDBx/mmJSON format | 2bn5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bn/2bn5 ftp://data.pdbj.org/pub/pdb/validation_reports/bn/2bn5 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 3796.136 Da / Num. of mol.: 1 / Fragment: B BOX, RESIDUES 651-683 Source method: isolated from a genetically manipulated source Source: (gene. exp.) DROSOPHILA MELANOGASTER (fruit fly) / Plasmid: PGEX / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q7JPS0 |
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#2: Protein/peptide | Mass: 2278.678 Da / Num. of mol.: 1 / Fragment: PSI BINDING REGION, RESIDUES 405-425 / Source method: obtained synthetically / Source: (synth.) DROSOPHILA MELANOGASTER (fruit fly) / References: UniProt: P17133 |
Sequence details | RESIDUES 651-683 ONLY RESIDUES 405-425 ONLY |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING 15N, 13C DOUBLE- LABELLED PROTEIN AND UNLABELLED PEPTIDE. DOUBLE-FILTERED 2D EXPERIMENTS WERE USED TO OBSERVE PEPTIDE RESONANCES SELECTIVELY |
-Sample preparation
Details | Contents: 93% H2O, 7% D2O |
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Sample conditions | Ionic strength: 100MM NACL, 10MM SODIUM PHOSPHATE / pH: 6.5 / Pressure: 1.0 atm / Temperature: 290.0 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: SIMULATED ANNEAING / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE | |||||||||
NMR ensemble | Conformer selection criteria: LOW NOE ENERGY / Conformers calculated total number: 50 / Conformers submitted total number: 28 |