deNEDDylase activity / protein deneddylation / regulation of proteolysis / protein neddylation / TGF-beta receptor signaling activates SMADs / anatomical structure morphogenesis / cysteine-type peptidase activity / post-translational protein modification / Iron uptake and transport / protein modification process ...deNEDDylase activity / protein deneddylation / regulation of proteolysis / protein neddylation / TGF-beta receptor signaling activates SMADs / anatomical structure morphogenesis / cysteine-type peptidase activity / post-translational protein modification / Iron uptake and transport / protein modification process / protein localization / modification-dependent protein catabolic process / protein tag activity / UCH proteinases / Cargo recognition for clathrin-mediated endocytosis / Neddylation / ubiquitin-dependent protein catabolic process / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / ubiquitin protein ligase binding / regulation of transcription by RNA polymerase II / proteolysis / extracellular exosome / nucleoplasm / nucleus / cytosol Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 214 / Source method: isolated from a natural source / Formula: H2O
Compound details
FUNCTION: POSSIBLE INVOLVEMENT IN THE RELEASE OF SENTRINS. POSSIBLE ROLE DURING THE EMBRYONIC ...FUNCTION: POSSIBLE INVOLVEMENT IN THE RELEASE OF SENTRINS. POSSIBLE ROLE DURING THE EMBRYONIC DEVELOPMENT AND DIFFERENTIATION OF THE CENTRAL NERVOUS SYSTEM
Sequence details
EXTRA N-TERMINAL RESIDUE FROM CLONING SER FOR CHAIN B. THE ORIGINAL SAMPLE FOR CHAIN B CONTAINS 82 ...EXTRA N-TERMINAL RESIDUE FROM CLONING SER FOR CHAIN B. THE ORIGINAL SAMPLE FOR CHAIN B CONTAINS 82 RESIDUES INCLUDING GLY GLY LEU ARG GLN (77-81) BUT THESE RESIDUES ARE CLEAVED OFF BY THE PROTEASE DURING THE COMPLEX FORMATION. THESE RESIDUES ARE NOT THEREFORE PART OF OF THE CRYSTALLIZED COMPLEX.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.3 Å3/Da / Density % sol: 46.1 %
Crystal grow
pH: 4.5 Details: CRYSTALS WERE GROWN BY SETTING-DROP METHOD BY MIXING THE NEDP1-NEDD8 COMPLEX (20MG/ML) WITH EQUAL VOLUME OF RESERVOIR SOLUTION CONTAINING 20%PEG8000, 200MM NACL, 100MM PHOSPHATE CITRATE PH4.5, pH 4.50