+Open data
-Basic information
Entry | Database: PDB / ID: 2b7j | ||||||
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Title | Crystal Structure of Yeast Sco1 with Copper Bound | ||||||
Components | SCO1 protein | ||||||
Keywords | METAL BINDING PROTEIN / Metallochaperone / Cytochrome c Oxidase / Sco / Sco1 | ||||||
Function / homology | Function and homology information copper chaperone activity / mitochondrial cytochrome c oxidase assembly / intracellular copper ion homeostasis / cell redox homeostasis / cellular response to oxidative stress / mitochondrial inner membrane / copper ion binding / mitochondrion Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Abajian, C. / Rosenzweig, A.C. | ||||||
Citation | Journal: J.Biol.Inorg.Chem. / Year: 2006 Title: Crystal structure of yeast Sco1. Authors: Abajian, C. / Rosenzweig, A.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2b7j.cif.gz | 136.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2b7j.ent.gz | 107.8 KB | Display | PDB format |
PDBx/mmJSON format | 2b7j.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b7/2b7j ftp://data.pdbj.org/pub/pdb/validation_reports/b7/2b7j | HTTPS FTP |
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-Related structure data
Related structure data | 2b7kSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 22905.924 Da / Num. of mol.: 4 / Fragment: residues 96-295 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: SCO1 / Plasmid: pET-3a / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta(DE3)pLysS / References: UniProt: P23833 #2: Chemical | ChemComp-CU / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 36.8 % |
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Crystal grow | Temperature: 291 K / pH: 5.3 Details: Lithium Sulfate, Sodium Citrate, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 5.30 |
-Data collection
Diffraction | Mean temperature: 113 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 5ID-B / Wavelength: 1.3768 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Mar 27, 2005 |
Radiation | Monochromator: GLASS / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.3768 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→81.65 Å / Num. obs: 24962 / % possible obs: 79 % / Observed criterion σ(I): 0 / Rsym value: 0.062 / Net I/σ(I): 9.6 |
Reflection shell | Resolution: 2.3→2.42 Å / Mean I/σ(I) obs: 2.5 / Rsym value: 0.3 / % possible all: 42.6 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2B7K Resolution: 2.3→81.65 Å / σ(F): 0 / Stereochemistry target values: ENGH & HUBER
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Displacement parameters | Biso mean: 44.1 Å2
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Refinement step | Cycle: LAST / Resolution: 2.3→81.65 Å
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Refine LS restraints |
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