[English] 日本語
Yorodumi- PDB-2azu: X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35*GL... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2azu | ||||||
---|---|---|---|---|---|---|---|
Title | X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35*GLN AND HIS35*LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA | ||||||
Components | AZURIN | ||||||
Keywords | ELECTRON TRANSFER(CUPROPROTEIN) | ||||||
Function / homology | Function and homology information transition metal ion binding / electron transfer activity / periplasmic space / copper ion binding / zinc ion binding / identical protein binding Similarity search - Function | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.9 Å | ||||||
Authors | Nar, H. / Messerschmidt, A. / Huber, R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1991 Title: X-ray crystal structure of the two site-specific mutants His35Gln and His35Leu of azurin from Pseudomonas aeruginosa. Authors: Nar, H. / Messerschmidt, A. / Huber, R. / van de Kamp, M. / Canters, G.W. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2azu.cif.gz | 110 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2azu.ent.gz | 90.4 KB | Display | PDB format |
PDBx/mmJSON format | 2azu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/az/2azu ftp://data.pdbj.org/pub/pdb/validation_reports/az/2azu | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 13935.825 Da / Num. of mol.: 4 / Mutation: H35L Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / References: UniProt: P00282 #2: Chemical | ChemComp-CU / #3: Chemical | ChemComp-NO3 / | #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
---|
-Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 46.8 % | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS Method: vapor diffusion / PH range low: 5.7 / PH range high: 5.5 | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Radiation | Scattering type: x-ray |
---|---|
Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 9999 Å / Num. obs: 33434 / Rmerge(I) obs: 0.077 |
-Processing
Software |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Rfactor Rwork: 0.17 / Highest resolution: 1.9 Å | ||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 1.9 Å
| ||||||||||||
Refine LS restraints |
| ||||||||||||
Refinement | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 8 Å / Num. reflection obs: 32548 / Rfactor obs: 0.17 | ||||||||||||
Solvent computation | *PLUS | ||||||||||||
Displacement parameters | *PLUS Biso mean: 20.5 Å2 | ||||||||||||
Refine LS restraints | *PLUS
|